Deciphering the internal mechanisms of ciprofloxacin affected anaerobic digestion, its degradation and detoxification mechanism

Ciprofloxacin (CIP) is widely used in livestock farms, but the internal mechanism of the effect of residual CIP in actual livestock wastewater on anaerobic digestion (AD) performance remains unknown. This study examined the dose specific effects of CIP (0.5-2 mg/L) on livestock wastewater AD by analyzing acidogenesis and methanogenesis. 0.5 mg/L CIP promoted methane production by facilitating acidogenesis and acetogenesis. Compared with the control, the cumulative methane production increased from 331.38 to 407.44 mL/g VS at a dose of 0.5 mg/L, an increase of 22.95 %. However, as the dose of CIP increased, the cumulative methane production gradually decreased to 217.64 mL/g VS (2 mg/L). Microbial community analysis revealed that CIP had the greatest impact on methane production by influencing the activity of acidogenic bacteria. Meanwhile, acidogenesis was critical for CIP degradation. In acidogenesis, hydroxylation, amination, defluorination, decarboxylation, and piperazine ring breaking not only degraded CIP but also reduced its toxicity. Therefore, a large number of intermediates could be continuously degraded by microorganisms. However, as the dosage of CIP increased, the ability of microorganisms to degrade intermediates decreased.

Automated summary

The concentration of CIP has dose-specific effects on methane production in livestock wastewater, with low doses promoting methane production and high doses inhibiting it. Microbial acidification and degradation are key processes in the impact of CIP on methane production.