The role of magnetite (Fe3O4) particles for enhancing the performance and granulation of anammox

In this study, two lab-scale sequencing batch reactors each with an effective volume of 2.3 L were operated as C-AMX (no carrier addition) and M-AMX (magnetite carrier added) for 147 days with synthetic wastewater at an NLR range of 0.19-0.47 kgN/m3/d. The long-term effect of magnetite on the granulation and performance of anammox bacteria in terms of nitrogen removal and other essential parameters were confirmed. In phase I (1-24 days), M-AMX took approx-imately 12 days to obtain a nitrogen removal rate (NRR) above 80 % of the initial input nitrogen. Although free nitrous acid inhibited the reactor at a high concentration at the onset of phase III, the NRR of M-AMX recovered about 3.7 times faster than that of C-AMX. In addition, it was confirmed that the M-AMX granules had a dense and compact struc-ture compared to C-AMX, and the presence of the carrier promoted the development of these resilient granules. While the measured microbial stress gradually increased in C-AMX reactor, a vice versa was observed in the M-AMX reactor as granulation proceeded. Compared to other alternative iron-based carrier particles, the stable crystal structure of magnetite as a carrier created a mechanism where filamentous bacteria groups were repelled from the granulation hence the microbial stress in the M-AMX in the final phase was 61.54 % lower than that in the C-AMX. The iron rich environment created by the magnetite addition led to Ignavibacteria, (a Feammox bacteria) increasing significantly in the M-AMX bioreactor.

Automated summary

In this study, the long-term effect of magnetite on the granulation and performance of anammox bacteria in the sequencing batch reactors was investigated. The addition of magnetite as a carrier promoted the development of compact and dense granules, leading to a faster recovery of nitrogen removal rate and lower microbial stress compared to the reactor without carrier addition. The presence of magnetite also created a stable crystal structure that repelled filamentous bacteria groups and allowed for a higher level of nitrogen removal efficiency.