ME-CAGEBIRDr,X-CPMG-HSQMBC. A phase sensitive, multiplicity edited long range HSQC with absorptive line shapes

ME-CAGEBIRD(r,X)-CPMG-HSMBC pulse sequence is a phase sensitive, carbon multiplicity edited 2D-experiment for detecting heteronuclear correlations originating from long-range H-1, C-13-couplings, (n)J(CH). The presented method allows measurement of (n)J(CH)-values as well as is capable of separating different carbon types in subspectra (C-13/(CH2)-C-13 and (CH)-C-13/(CH3)-C-13) with minimal amount of cross talk i.e. cross peaks from wrong carbon multiplicity. Pure lineshapes and clean subspectra are achieved by utilizing CPMG in polarization transfer period, CRISIS-approach in multiplicity editing period and zero-quantum filtration. The obtained spectral properties together with simple setup of the experiment make ME-CAGEBIRD(r,X)-CPMG-HSMBC a useful addition into synthetic organic chemistry oriented NMR-tool collection. (C) 2016 Elsevier Inc. All rights reserved.