4.3 Article

Characterisation of P2Y receptor subtypes mediating vasodilation and vasoconstriction of rat pulmonary artery using selective antagonists

期刊

PURINERGIC SIGNALLING
卷 18, 期 4, 页码 515-528

出版社

SPRINGER
DOI: 10.1007/s11302-022-09895-x

关键词

AR-C118925XX; P2Y(2) receptor; Pulmonary artery; Vasoconstriction; Vasodilation

资金

  1. British Heart Foundation [FS/04/070]

向作者/读者索取更多资源

This study investigated the roles of individual P2Y receptor subtypes in nucleotide-induced pulmonary vasodilation and vasoconstriction. The results showed that UTP causes pulmonary vasodilation via P2Y(2) receptors, UDP acts on P2Y(6) receptors, and ADP acts on P2Y(1) receptors. The mechanism of ATP-induced vasodilation remains unclear.
Pulmonary vascular tone is modulated by nucleotides, but which P2 receptors mediate these actions is largely unclear. The aim of this study, therefore, was to use subtype-selective antagonists to determine the roles of individual P2Y receptor subtypes in nucleotide-evoked pulmonary vasodilation and vasoconstriction. Isometric tension was recorded from rat intrapulmonary artery rings (i.d. 200-500 mu m) mounted on a wire myograph. Nucleotides evoked concentration- and endothelium-dependent vasodilation of precontracted tissues, but the concentration-response curves were shallow and did not reach a plateau. The selective P2Y(2) antagonist, AR-C118925XX, inhibited uridine 5'-triphosphate (UTP)- but not adenosine 5'-triphosphate (ATP)-evoked relaxation, whereas the P2Y(6) receptor antagonist, MRS2578, had no effect on UTP but inhibited relaxation elicited by uridine 5'-diphosphate (UDP). ATP-evoked relaxations were unaffected by the P2Y(1) receptor antagonist, MRS2179, which substantially inhibited responses to adenosine 5'-diphosphate (ADP), and by the P2Y(12/13) receptor antagonist, cangrelor, which potentiated responses to ADP. Both agonists were unaffected by CGS1593, an adenosine receptor antagonist. Finally, AR-C118925XX had no effect on vasoconstriction elicited by UTP or ATP at resting tone, although P2Y(2) receptor mRNA was extracted from endothelium-denuded tissues using reverse transcription polymerase chain reaction with specific oligonucleotide primers. In conclusion, UTP elicits pulmonary vasodilation via P2Y(2) receptors, whereas UDP acts at P2Y(6) and ADP at P2Y(1) receptors, respectively. How ATP induces vasodilation is unclear, but it does not involve P2Y(1), P2Y(2), P2Y(12), P2Y(13), or adenosine receptors. UTP- and ATP-evoked vasoconstriction was not mediated by P2Y(2) receptors. Thus, this study advances our understanding of how nucleotides modulate pulmonary vascular tone.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据