Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli

CCP6 is a member of cytosolic carboxypeptidases (CCPs) family, an eraser of a reversible protein post translational modification - polyglutamylation, and represents a potential therapeutic target. Currently, production of CCPs mainly depends on eukaryotic expression system, which is time-consuming and costly. Here, we reported that mouse origin full-length CCP6 can be successfully expressed in the soluble fraction of bacteria ArcticExpress (DE3) strain. However, the recombinant mCCP6 was initially co-purified with Cpn60 in a stoichiometric ratio of roughly 1:7 and exhibited no enzyme activity. When coupled with a step to promote the release of the substrate protein from the chaperonins by treatment with ATP/Mg2+/K+, the recombinant CCP6 with deglutamylation activity was obtained, though still partially associated with Cpn60. This is the first report, to our knowledge, that the successful expression and purification of active recombinant mammalian CCPs using a bacterial system was achieved.

Automated summary

This study successfully expressed and purified active recombinant mouse CCP6 in a bacterial system with deglutamylation activity. Despite initial association with Cpn60, the CCP6 obtained after proper treatment exhibited enzyme activity.