Phenolic content and antioxidant activity of Ophiocordyceps Sobolifera extract for renal injury prevention

In vitro and in vivo antioxidant activities of the Vietnam-grown Ophiocordyceps sobolifera were investigated for the first time. The contents of phenolic and flavonoid compounds found in O. sobolifera were 18.08 +/- 0.52 mg GAE/g dry weight and 10.48 +/- 0.02 mg QUE/g dry weight, respectively, and the total antioxidant capacity of the extracts was equivalent to 18.25 +/- 0.15 mg GA/g dry weight or 10.24 +/- 0.03 mg AS/g dry weight. At concentration 2000 mu g/mL, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of water extracts from Ophiocordyceps sobolifera was 80 %. The amounts of gallic acid, quercetin, quercitrin, and hesperidin dominated the extracts at 193.60 +/- 0.02; 142.07 +/- 0.02; 544.53 +/- 0.03 and 110.08 +/- 0.01 mu g/g, respectively, out of a total of 990.27 mu g/g dry weight active phenolic fraction. In the in vivo assays, the Ophiocordyceps sobolifera extract showed its ability in protecting kidneys of rats induced injury by cisplatin via decreasing levels of creatinine (47.7 %) and Blood urea nitrogen (BUN) (25.53 %) in blood plasma and malondialdehyde (MDA) (52.41 %) level in renal tissue. The findings of this study provided evidence that Ophiocordyceps sobolifera is a promising resource of natural antioxidants.

Automated summary

The antioxidant activities of Ophiocordyceps sobolifera grown in Vietnam were investigated for the first time, both in vitro and in vivo. The results showed that O. sobolifera extracts exhibited strong antioxidant capacity and could protect against kidney injury in rats induced by cisplatin. The phenolic and flavonoid compounds found in O. sobolifera were identified and their concentrations were determined, indicating the potential of this fungus as a natural source of antioxidants.