4.8 Article

Cholesterol inhibits human voltage-gated proton channel hHv1

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Summary: This article investigates the conformational dynamics and gating mechanisms of voltage-gated proton (Hv) channels. By using single-molecule fluorescence resonance energy transfer (smFRET) with purified human voltage-gated proton (hHv1) channels reconstituted in liposomes, the real-time conformational trajectories of the S4 segment were observed. The results demonstrate that voltage and pH gradient can shift the conformational dynamics of the S4 segment, controlling the gating of the channel. The authors propose a kinetic model to explain the voltage and pH gating mechanisms in Hv channels, which may have implications for understanding the voltage sensing and gating in other voltage-gated ion channels.
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