GFP fusion promotes the soluble and active expression of a pea actin isoform (PEAc1) in Escherichia coli

Article Biochemical Research Methods

Optimized expression of large fragment DNA polymerase I from Geobacillus stearothermophilus in Escherichia coli expression system

Eva Agustriana et al.

Summary: This study focused on the scaling-up production and optimization of expression conditions for Bst DNA polymerase, offering a feasible noncommercial enzyme option for cost-effective detection of COVID-19.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2023)

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Editorial Material Biochemistry & Molecular Biology

Connecting the dots: Membrane nanodomains mediate clustering of actin-nucleator Type I formins in Arabidopsis immune responses

Tian Zhang

PLANT CELL (2022)

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Article Biochemistry & Molecular Biology

Membrane nanodomains modulate formin condensation for actin remodeling in Arabidopsis innate immune responses

Zhiming Ma et al.

Summary: This study demonstrates that the condensation of nanodomain constituents and the self-assembly of remorin proteins regulate the condensation and activity of formins during innate immunity in Arabidopsis thaliana. Through interaction with formins, remorin enhances actin nucleation, influencing the transduction of immune signals. Such nanodomain- and remorin-mediated regulation is expected to be a common feature in plant innate immune responses, creating spatially separated biochemical compartments and fine-tuning membrane properties for immune signal transduction in the host.

PLANT CELL (2022)

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Review Biotechnology & Applied Microbiology

Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications

Arshpreet Bhatwa et al.

Summary: Recombinant proteins are crucial in industrial applications, with E. coli being the primary bacterial host for their production. The challenge of inclusion body formation can be overcome through various strategies, such as adjusting growth conditions and modifying proteins of interest. Understanding the unique features and formation mechanism of inclusion bodies will help maximize their potential advantages.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

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Article Chemistry, Multidisciplinary

Soluble Expression of Small Antibody Fragments against PD-L1 Using Escherichia coli with High Yield and Purity

Sun-Hee Kim et al.

Summary: PD-L1, a surface protein overexpressed in tumor cells, has attracted attention for targeted therapy. Researchers successfully expressed and purified small antibody fragments against PD-L1 using Escherichia coli, suggesting their potential use as new agents for blocking or detecting PD-L1.

APPLIED SCIENCES-BASEL (2021)

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Article Biochemistry & Molecular Biology

PredictProtein - Predicting Protein Structure and Function for 29 Years

Michael Bernhofer et al.

Summary: PredictProtein has been a one-stop online resource for protein sequence analysis since 1992, providing various predictions including protein structure, function, and binding. Recently, new prediction methods such as deep learning embeddings and prediction of protein and residues binding DNA, RNA, or other proteins have been added to enhance its usability for computational and experimental biologists.

NUCLEIC ACIDS RESEARCH (2021)

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Article Biochemistry & Molecular Biology

Actin Isovariant ACT7 Modulates Root Thermomorphogenesis by Altering Intracellular Auxin Homeostasis

Sumaya Parveen et al.

Summary: The study demonstrates the important role of the vegetative class actin isovariant ACT7 in regulating the response of Arabidopsis roots to moderate to high temperatures. Loss of ACT7 resulted in increased inhibition of root elongation, reduced cell production rate and cell elongation, and altered dynamics of actin in response to prolonged moderate to high temperatures. This suggests that ACT7 modulates the long-term response of Arabidopsis roots to moderate to high temperatures.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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Article Biochemistry & Molecular Biology

Studies on the Structure and Properties of Membrane Phospholipase A1 Inclusion Bodies Formed at Low Growth Temperatures Using GFP Fusion Strategy

Svetlana Bakholdina et al.

Summary: The study showed that cultivation temperature influences the conformational quality of Yersinia pseudotuberculosis phospholipase A(1) (PldA) in inclusion bodies, with optimal fluorescence and expression observed at 18 degrees C. Solubilization of PldA-GFP from inclusion bodies in urea and SDS leads to denaturation, revealing structural heterogeneity. The use of GFP as a fusion partner improves conformational quality but negatively impacts enzymatic activity.

MOLECULES (2021)

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Review Biotechnology & Applied Microbiology