期刊
POSTHARVEST BIOLOGY AND TECHNOLOGY
卷 192, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.postharvbio.2022.112019
关键词
Phenolic compounds; Active antifungal ingredients; Colletotrichum gloeosporioides; Walnut; The mixed antifungal agents
资金
- Improved Variety Program of Shandong Province [2020LZGC0902, 2017LZGC019]
- Shandong Academy of Agricultural Sciences, Introduction and Training of High-level Talents [11200214442049]
- Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties [2021C02070-1]
- Zhejiang A & F University Research and Development Fund Talent Startup Project [2021LFR013]
This study investigated the phenolic compounds related to walnut anthracnose resistance and found that some of these compounds have antifungal activity against the pathogen. These findings provide a basis for further research on controlling anthracnose disease.
Phenolic compounds play important roles in pharmacology, nutrition and inhibition of pathogens. However, there are few studies on the identification of phenolic compounds related to walnut anthracnose resistance. In this study, we examined the walnut cultivars 'Amigo' and 'HuoTe', which are resistant and susceptible to anthracnose (Colletotrichum gloeosporioides), respectively. After inoculation with C. gloeosporioides, the overlapping differentially expressed genes of 'Amigo' and 'HuoTe' were mainly enriched in the phenolic compound biosynthesis pathway, and the genes related to chorismate and phenylalanine upstream metabolism pathways were induced. The phenolic compounds in the husks of 'Amigo' and 'HuoTe' were mainly phenylpropanoidsphenolic acids, benzoic acid derivatives-phenolic acids, procyanidins and flavonols. Further analysis revealed that 11 phenolic compounds were putatively related to the response to C. gloeosporioides. Subsequently, we conducted sensitivity tests with these potential active ingredients and found that 4-coumaric acid and p- hydroxybenzoic acid inhibited C. gloeosporioides growth and can be used as effective antifungal agents. The minimum inhibitory concentrations (MICs) of 4-coumaric acid and p-hydroxybenzoic acid were 1 g L-1 and 2 g L-1, respectively. The mixed antifungal agents composed of 4-coumaric acid and p-hydroxybenzoic acid had an obvious inhibitory effect on C. gloeosporioides in walnut fruit with an MIC ratio of 1:2. These results reveal the induced phenolic compounds of walnut husk and their active ingredients in response to anthracnose and provide a basis for further research on regulating and controlling anthracnose disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据