Sensing of nucleosides, nucleotides and DNA using luminescent Eu complex by normal and time resolved fluorescence techniques

The interaction of Eu-1,4,7,10-tetraazacyclododecane (Cyclen) complex by using 4,4,4 trifluoro-1-(2-naphthyl)1,3-butanedione (TNB) as antenna with some nucleosides (guanosine, adenosine, cytidine and inosine), nucleotides (AMP, GMP, CMP, ATP and IMP) and DNA is studied using fluorescence technique. Two detection modes are employed one is the time-resolved mode, and the other is the normal luminescence mode. The time-resolved mode is more sensing than the normal luminescence mode in the present study. By using Benesi-Hildebrand equation binding constants were determined at various temperatures. Thermodynamic parameters showed that the reaction is spontaneous through the obtained negative values of free energy change Delta G. The enthalpy Delta H and the entropy Delta S of reactions were all determined. (C) 2015 Elsevier B.V. All rights reserved.