Gamma (γ)-radiation stress response of the cyanobacterium Anabaena sp. PCC7120: Regulatory role of LexA and photophysiological changes

High radioresistance of the cyanobacterium, Anabaena sp. PCC7120 has been attributed to efficient DNA repair, protein recycling, and oxidative stress management. However, the regulatory network involved in these batteries of responses remains unexplored. In the present study, the role of a global regulator, LexA in modulating gamma (gamma)-radiation stress response of Anabaena was investigated. Comparison of the cytosolic proteome profiles upon gamma-radiation in recombinant Anabaena strains, AnpAM (vector-control) and AnlexA(+) (LexA-overexpressing), revealed 41 differentially accumulated proteins, corresponding to 29 distinct proteins. LexA was found to be involved in the regulation of 27 of the corresponding genes based on the presence of AnLexA-Box, EMSA, and/or qRT-PCR studies. The majority of the regulated genes were found to be involved in C-assimilation either through photosynthesis or C-catabolism and oxidative stress alleviation. Photosynthesis, measured in terms of PSII photophysiological parameters and thylakoid membrane proteome was found to be affected by gamma-radiation in both AnpAM and AnlexA(+) cells, with LexA affecting them even under control growth conditions. Thus, LexA functioned as one of the transcriptional regulators involved in modulating gamma-radiation stress response in Anabaena. This study could pave the way for a deeper understanding of the regulation of gamma-radiation-responsive genes in cyanobacteria at large.

Automated summary

This study revealed the role of a global regulator, LexA, in modulating the gamma-radiation stress response of the cyanobacterium Anabaena. Proteome analysis of Anabaena strains subjected to gamma-radiation showed differential accumulation of 41 proteins, corresponding to 29 distinct proteins, with LexA regulating 27 of the corresponding genes. The regulated genes were mainly involved in photosynthesis, carbon assimilation, and oxidative stress alleviation. The study also found that LexA influenced photosynthesis and thylakoid membrane proteome even under normal growth conditions, indicating its role in stress response management.