期刊
PLANT JOURNAL
卷 112, 期 2, 页码 399-413出版社
WILEY
DOI: 10.1111/tpj.15951
关键词
cell wall modification; ethylene; plant hormone; non-coding RNAs; DNA (5mC) methylation; RNA (m5C) methylation; tomato fruit; target gene
资金
- National Natural Science Foundation of China [32072284]
- Special Innovation Ability Construction Fund of Beijing Academy of Agricultural and Forestry Sciences [20210402, 20210437, 20200427]
- Collaborative Innovation Center of Beijing Academy of Agricultural and Forestry Sciences [201915]
- China Agriculture Research System of MOF and MARA [CARS-23]
This study identified mRNAs with increasing m5C levels involved in fruit ripening, as well as genes associated with response to biotic and abiotic stresses and cell wall metabolism. Differential expression of genes encoding transcription factors and key enzymes related to ethylene biosynthesis and signal transduction was also observed. These findings provide important insights into the mechanisms of fruit ripening and post-harvest behavior.
Ripening is the last, irreversible developmental stage during which fruit become palatable, thus promoting seed dispersal by frugivory. In Alisa Craig fruit, mRNAs with increasing m5C levels, such as STPK and WRKY 40, were identified as being involved in response to biotic and abiotic stresses. Furthermore, two mRNAs involved in cell wall metabolism, PG and EXP-B1, also presented increased m5C levels. In the Nr mutant, several m5C-modified mRNAs involved in fruit ripening, including those encoding WRKY and MADS-box proteins, were found. Targets of long non-coding RNAs and circular RNAs with different m5C sites were also found; these targets included 2-alkenal reductase, soluble starch synthase 1, WRKY, MADS-box, and F-box/ketch-repeat protein SKIP11. A combined analysis of changes in 5mC methylation and mRNA revealed many differentially expressed genes with differentially methylated regions encoding transcription factors and key enzymes related to ethylene biosynthesis and signal transduction; these included ERF084, EIN3, AP2/ERF, ACO5, ACS7, EIN3/4, EBF1, MADS-box, AP2/ERF, and ETR1. Taken together, our findings contribute to the global understanding of the mechanisms underlying fruit ripening, thereby providing new information for both fruit and post-harvest behavior.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据