4.7 Article

Heilaohuacid G, a new triterpenoid from Kadsura coccinea inhibits proliferation, induces apoptosis, and ameliorates inflammation in RA-FLS and RAW 264.7 cells via suppressing NF-κB pathway

期刊

PHYTOTHERAPY RESEARCH
卷 36, 期 10, 页码 3900-3910

出版社

WILEY
DOI: 10.1002/ptr.7527

关键词

Heilaohu; Kadsura coccinea; Rheumatoid arthritis; Schisandraceae; Triterpenoid; Tujia ethnomedicine

资金

  1. Changjiang Scholars Program in Ministry Education [T2019133]
  2. National Natural Science Foundation of China [82174078, 81803708, 81874369, 82074122]

向作者/读者索取更多资源

The study found that Heilaohuacid G can treat rheumatic arthritis by inhibiting the NF-kappa B pathway, thus inhibiting inflammatory reactions and the proliferation of RA-FLS cells.
Heilaohu, the roots of Kadsura coccinea, has been used in Tujia ethnomedicine to treat rheumatic arthritis (RA). Heilaohuacid G (1), a new 3,4-seco-lanostane type triterpenoid isolated from the ethanol extract of Heilaohu, whose structure was determined using HR-ESI-MS data, NMR spectroscopic analyses, and ECD calculations. In this study, our purpose is to elucidate the mechanisms of Heilaohuacid G in the treatment of RA by inhibited proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells and inhibited the inflammatory reactions in LPS-induced RA-FLS and RAW 264.7 cell lines via inhibiting NF-kappa B pathway. The biological activity screening experiments indicated that Heilaohuacid G significantly inhibited proliferation of RA-FLS cells with IC50 value of 8.16 +/- 0.47 mu M. CCK-8 assay, ELISA, flow cytometry assay, and Western blot were used to measure the changes of cell viability, apoptosis, and the release of inflammatory cytokines. Heilaohuacid G was found not only induced RA-FLS cell apoptosis, but also inhibited the inflammatory reactions in LPS-induced RA-FLS and RAW 264.7 cell lines via inhibiting NF-kappa B pathway. Furthermore, Heilaohuacid G (p.o.) at doses of 3.0, 6.0, and 12.0 mg/kg and the ethanol extracts of Heilaohu (p.o.) at doses of 200, 400, and 800 mg/kg both were confirmed antiinflammatory effects on xylene-induced ear mice edema model.

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