期刊
PHYTOMEDICINE
卷 106, 期 -, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2022.154415
关键词
Perillyl alcohol; anti-inflammatory; chronic restraint stress; neuroinflammation; dextran sulfate sodium; ulcerative colitis
资金
- Department of Pharmaceuticals
- Ministry of chemical and fertilizers, Government of India
- Ministry of Defence, Government of India [O/o DG (TM) /81/48222/LSRB-335/BTB/2018]
This study explored the anti-ulcerative properties of perillyl alcohol (POH) on stress aggravated ulcerative colitis. The results showed that POH reversed oxidative stress and inflammatory response by inhibiting TLR4/NF-kB pathway and IL-6/JAK2/STAT3 pathway, leading to a decrease in pro-inflammatory cytokines. It also improved the physiological and histological changes of ulcerative colitis, and increased the mobility time and neurotransmitters while decreasing cortisol in mice brains.
Background: Ulcerative colitis (UC) is the most prevalent chronic inflammatory immune bowel disease. The modernization of lifestyle accompanied by the stress to cope with the competition has resulted in a new range of complications where stress became a critical contributing factor for many diseases, including UC. Hence there is an urgent need to develop a dual role in curtailing both systemic and neuroinflammation. Perillyl alcohol (POH) is a natural essential oil found in lavender, peppermint, cherries etc and has been widely studied for its strong anti-inflammatory, antioxidant and anti-stress properties. Hypothesis/Purpose: POH regulates the various inflammatory signaling cascades involved in chronic inflammation by inhibiting farnesylransferase enzyme. Several studies reported that POH could inhibit the phosphory- lation of NF-kappa B, STAT3 and promote the endogenous antioxidant enzymes like Nrf2 via farnesyltransferase enzyme inhibition. Also, the effects of POH against UC is not known yet. Thus, this study aims to explore the anti-ulcerative properties of POH on stress aggravated ulcerative colitis in C57BL/6 mice. Methods: Ulcerative colitis was induced by duel exposure of chronic restraint stress (day 1 to day 28) and 2.5% dextran sulphate sodium (day8 to day14) in mice. POH treatment 100 and 200 mg/kg was administred from day14 ti day28 following oral route of administration. Disease activity index, colonoscopy, western blot analysis and histological analysis, neurotransmitter analysis and Gene expression studies were perofomerd to asses the anti-colitis effects of POH. Results: The treatment reversed the oxidative stress and inflammatory response by inhibiting TLR4/NF-kB pathway, and IL-6/JAK2/STAT3 pathway in both isolated mice colons and brains. The inhibition of these pathways resulted in a decrease in pro-inflammatory cytokines like IL-6, IL-1 beta and TNF-alpha. The treatment improved the physiological and histological changes with decreased ulcerations as examined by colonic endoscopy and Haematoxylin and Eosin staining. The treatment also improved the behavior response as it increased mobility time which was reduced by chronic restrained stress. This was due to increased satiety neurotransmitters like dopamine and semtonin and decreased cortisol in mice brains. Conclusion: These results infer that POH has significant anti-colitis activity on chronic restraint stress aggravated DSS-induced UC in mice.
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