Furostanol saponins from Asparagus racemosus as potential hypoglycemic agents

Bioactivity guided phytochemical investigation led to isolation of six undescribed furostanol saponins, furoasparoside A-F along with five known compounds, gallic acid, methyl gallate, quercetin-3-O-beta-glucopyranoside, liquiritigenin 4' -O-beta-apiofuranosyl-(1. 2)-beta-glucopyranoside and beta-glucogallin for the first time from the roots of Asparagus racemosus. Isolated saponins were screened for their antidiabetic potential in L6-GLUT4myc myotubes in vitro followed by an in vivo evaluation in streptozocin-induced diabetic rats and db/db mice. Furoasparoside E produced a notable decrease in the postprandial blood glucose profile, in leptin receptor-deficient db/db mice, type 2 diabetes model. The effect of furoasparoside E on GLUT4 translocation was found to be mediated by the AMPK-dependent signaling pathway in L6-GLUT4myc myotubes. Moreover, it emerged as a stable plant metabolite with higher bioavailability and efficacy in in vivo pharmacokinetic studies. Therefore, these studies indicated that furoasparoside E may serve as a propitious lead for the management of type 2 diabetes and its secondary complications from natural source.

Automated summary

Bioactivity guided phytochemical investigation led to the discovery of six new furostanol saponins from the roots of Asparagus racemosus. Among them, furoasparoside E showed significant potential in reducing blood glucose levels and may serve as a lead compound for the management of type 2 diabetes.