期刊
PHYTOCHEMICAL ANALYSIS
卷 34, 期 1, 页码 67-75出版社
WILEY
DOI: 10.1002/pca.3180
关键词
ligand fishing; magnetic nanoparticles; monoamine oxidase B; Parkinson's disease; Platycodon grandiflorum
This study aimed to screen and identify MAO-B inhibitors from the roots of Platycodon grandiflorus using MAO-B functionalised MNPs, HPLC-MS, and NMR analysis. Two inhibitors, protocatechuic aldehyde and coumarin, were identified with potential anti-PD activity, especially protocatechuic aldehyde that also increased cell viability. The results shed light on the anti-PD activity of Platycodon grandiflorus roots and the efficacy of using ligand fishing method for discovering MAO-B inhibitors.
Introduction As a famous traditional Chinese medicine, roots of Platycodon grandiflorus (Jacq.) A.DC. have shown multiple effects against neurodegenerative diseases. To investigate the components against Parkinson's disease (PD), the roots of P. grandiflora were selected as the research subject. Objective Screening and identifying of monoamine oxidase B (MAO-B) inhibitors from the roots of P. grandiflorum via enzyme functionalised magnetic nanoparticles (MNPs)-based ligand fishing combined with high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis. Method MAO-B functionalised MNPs have been synthesised for screening MAO-B inhibitors from the roots of P. grandiflorum. The ligands were identified by HPLC-MS and nuclear magnetic resonance (NMR) analysis, and their anti-PD activity was evaluated via MAO-B inhibition assay and cell viability assay in vitro. Results Two MAO-B inhibitors were fished out and identified by HPLC-MS as protocatechuic aldehyde (1) and coumarin (2), with the half maximal inhibitory concentrations of 28.54 +/- 0.39 and 25.39 +/- 0.29 mu M, respectively. Among them, 1 could also significantly increase the viability of 6-hydroxydopamine-damaged PC12 cells. Conclusion The results are helpful to elucidate the anti-PD activity of the plant, and the ligand fishing method has shown good potential in discovery of MAO-B inhibitors.
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