期刊
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
卷 27, 期 8, 页码 892-903出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10837450.2022.2128819
关键词
Polymeric lipid-core nanocapsules; Caryocar brasiliense Cambess; artemether; human glioblastoma; cell viability; antioxidant status
资金
- Cordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- CAPES Fellow (Bolsa de Mestrado)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Universidade Federal de Mato Grosso (UFMT)
- Fundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT)
The study found that lipid-core nanocapsules containing pequi oil as the oily core could improve the pharmacological action of artemether and potentially be used as a therapeutic agent for glioblastoma.
Glioblastomas are tumors that present a high mortality rate. Artemether (ART) is a lactone with antitumor properties, demonstrating low bioavailability and water solubility. In the present study, we developed lipid-core nanocapsules (LNC) containing pequi oil (Caryocar brasiliense Cambess) as the oily core for ART-loaded LNCs (LNCART) and evaluated their effect on human glioblastoma cells (U-87 MG). LNCs were developed by interfacial deposition of a preformed polymer, followed by physicochemical characterization. LNCART revealed a diameter of 0.216 mu m, polydispersity index of 0.161, zeta potential of -12.0 mV, and a pH of 5.53. Furthermore, mitochondrial viability, proliferation, total antioxidant status, and antioxidant enzyme activity were evaluated. ART reduced cell viability after 24 h and proliferation after 48 h of treatment at concentrations equal to or above 40 mu g . mL(-1). LNCART, at 1.25 mu g . mL(-1), reduced these parameters after 24 h of treatment. Furthermore, superoxide dismutase (SOD) activity was elevated, while glutathione reductase (GR) activity was reduced. These findings suggest that ART loaded into LNC may be a promising alternative to improve its pharmacological action and possible application as a therapeutic agent for glioblastoma.
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