LncRNA KCNQ1OT1 accelerates ovarian cancer progression via miR-125b-5p/CD147 axis

Background: Ovarian cancer (OC) is one of the most common gynecological malignancies with a high incidence. Researches showed that lncRNA KCNQ1OT1 (KCNQ1OT1) was involved various tumors progression, including OC. However, the precise mechanism of KCNQ1OT1 in OC needs to be further clarified. Objective: For investigate the underlying mechanism of KCNQ1OT1 regulating OC progression. Methods: CCK-8 assay, colony formation assay, Transwell assay, Western blot and quantitative real-time PCR (qRT-PCR) were performed to examine viability, proliferation, migration and invasion, genes and proteins' level. To identify KCNQ1OT1 as a regulator of miR-125b-5p and miR-125b-5p as a regulator of CD147, we used miRNA target prediction algorithms, Pearson's correlation analysis and dual-luciferase reporter gene assay. Results: KCNQ1OT1 was high expression and miR-125b-5p was low expression in OC, and KCNQ1OT1 was negatively correlated with that of miR-125b-5p in OC specimens. KCNQ1OT1 promoted OC cell proliferation and metastasis by binding to miR-125b-5p. miR-125b-5p targeted CD147, and which was negatively correlated with that of miR-125b-5p in OC specimens. KCNQ1OT1 was positively correlated with that of CD147 in OC specimens, and KCNQ1OT1 accelerated OC progression via miR-125b-5p/CD147 axis. Conclusion: KCNQ1OT1 accelerated OC progression via miR-125b-5p/CD147 axis indicating KCNQ1OT1 serve as a novel biomarker for OC treatment. Our research provides a new direction for OC treatment.

Automated summary

KCNQ1OT1 is highly expressed while miR-125b-5p is lowly expressed in ovarian cancer (OC). KCNQ1OT1 promotes OC cell proliferation and metastasis by binding to miR-125b-5p. MiR-125b-5p targets CD147, and its expression is negatively correlated with that of miR-125b-5p in OC specimens. KCNQ1OT1 is positively correlated with CD147 in OC specimens, and it accelerates OC progression through the miR-125b-5p/CD147 axis.