期刊
PARASITES & VECTORS
卷 15, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13071-022-05497-z
关键词
Toxoplasma gondii; Epitope vaccine; Bioinformatics; Human leukocyte antigen
资金
- National Key Research and Development Project [2018YFE0114500]
- National Natural Science Foundation of China [82072306, 81970746, 31571244]
- Hunan Provincial Natural Sciences Foundation [2022JJ30797]
This study identified a novel group of Toxoplasma membrane protein epitopes through genome-wide screening, which is important for the development of an effective vaccine against Toxoplasma infection.
Background: There is presently no effective and safe vaccine for Toxoplasma gondii for humans. The study described here was designed to search for a novel group of optimal B cell and T cell epitopes from Toxoplasma membrane proteins using genome-wide comprehensive screening. Methods: The amino acid sequences of membrane proteins of T. gondii were obtained from the UniProt database. The ABCPred and BepiPred servers were employed to predict the linear B cell epitopes. The Immune Epitope Database (IEDB) online service was utilized to forecast T cell epitopes within T. gondii membrane proteins that bind to human leukocyte antigen (HLA) class I (HLA-I) or HLA-II molecules. Results: From the 314 membrane proteins of T. gondii, a total of 14 linear B cell epitopes embedded in 12 membrane proteins were identified. Eight epitopes for major histocompatibility complex (MHC) class I (MHC-I) molecules and 18 epitopes for MHC-II molecules were ultimately selected, for which world population coverage percentiles were 71.94% and 99.76%, respectively. The top rated combinations of linear B cell epitopes and T cell epitopes covering both BALB/c mice and a majority of the human population were identified for the development of a protective vaccine. Conclusions: The ultimate vaccine construct described here, which comprises B cells, MHC-I and MHC-II epitopes, might protect individuals against T. gondii infection by inducing humoral and cellular immune responses. Graphic abstract
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据