期刊
ONCOGENE
卷 41, 期 40, 页码 4537-4546出版社
SPRINGERNATURE
DOI: 10.1038/s41388-022-02366-y
关键词
-
资金
- National Natural Science Foundation of China [82002220]
Zinc finger protein 154 (ZNF154) is hypermethylated at the promoter in esophageal squamous carcinoma (ESCC), leading to downregulation of the tumor suppressor gene and promoting cell proliferation and migration. Epigenetic editing using CRISPR/dCas9 technology can reverse the hypermethylation and inhibit ESCC cells. Furthermore, ZNF154 acts as a transcription factor to upregulate the expression of ESCC-associated tumor suppressor genes.
Zinc finger protein 154 (ZNF154) is hypermethylated at the promoter in many epithelial-derived solid tumors. However, its methylation status and function in esophageal squamous carcinoma (ESCC) are poorly understood. We found that the ZNF154 promoter is hypermethylated in ESCC and portends poor prognosis. In addition, ZNF154 functions as a tumor suppressor gene (TSG) in ESCC, and is downregulated by promoter hypermethylation. We established a targeted demethylation strategy based on CRISPR/dCas9 technology and found that the hypermethylation of ZNF154 promoter repressed ZNF154 induction, which in turn promoted the proliferation and migration of ESCC cells in vitro and in vivo. Finally, high-throughput CUT&Tag analysis, GEPIA software and qPCR were used to revealed the role of ZNF154 as a transcription factor to upregulate the expression of ESCC-associated tumor suppressor genes. Taken together, hypermethylation of the ZNF154 promoter plays an important role in the development of ESCC, and epigenetic editing is a promising tool for inhibiting ESCC cells with aberrant DNA methylation.
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