期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 100, 期 1, 页码 241-247出版社
WILEY
DOI: 10.1189/jlb.5TA0715-310RR
关键词
neutrophil; migration; inbred lines
资金
- U.S. National Institutes of Health [GM092804]
- Shriners Burns Institute [8700]
- Defense Advanced Research Projects Agency (DARPA) [W911NF-13-1]
Animal models of human disease differ in innate immune responses to stress, pathogens, or injury. Precise neutrophil phenotype measurements could facilitate interspecies comparisons. However, such phenotype comparisons could not be performed accurately with the use of current assays, as they require the separation of neutrophils from blood using species-specific protocols, and they introduce distinct artifacts. Here, we report a microfluidic technology that enables robust characterization of neutrophil migratory phenotypes in a manner independent of the donor species and performed directly in a droplet of whole blood. The assay relies on the particular ability of neutrophils to deform actively during chemotaxis through microscale channels that block the advance of other blood cells. Neutrophil migration is measured directly in blood, in the presence of other blood cells and serum factors. Our measurements reveal important differences among migration counts, velocity, and directionality among neutrophils from 2 common mouse strains, rats, and humans.
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