期刊
NEUROTOXICITY RESEARCH
卷 40, 期 6, 页码 1812-1823出版社
SPRINGER
DOI: 10.1007/s12640-022-00596-2
关键词
C.Elegans; Chemical exposure; Diuron; Toxicity; Pesticides
资金
- Sao Paulo Research Foundation (FAPESP) [2017/25402-5]
- Coordination for the Improvement of Higher Education Personnel (CAPES) [88887.467311/2019-00]
This study evaluated the toxic mechanisms of diuron and its metabolites using the C. elegans model. The results showed that exposure to high concentrations of these chemicals increased lethality and impaired ATP levels and dopaminergic neurodegeneration. The study also suggested the potential induction of autophagy as an adaptive survival mechanism.
The toxicity of diuron herbicide and its metabolites has been extensively investigated; however, their precise toxic mechanisms have yet to be fully appreciated. In this context, we evaluated the toxic mechanism of diuron, 3,4-dichloroaniline (DCA) and 3-(3,4-dichlorophenyl)-1-methylurea (DCPMU), using Caenorhabditis elegans (C. elegans) in the L1 larval stage. For this purpose, worms were acutely exposed to the test chemicals with a preliminary concentration range of 0.5 to 500 mu M and first analyzed for lethality (%). Next, the highest concentration (500 mu M) was considered for survival (%), reactive oxygen and nitrogen species (RONS), glutathione (GSH) and ATP levels, autophagy index, behavior, and dopaminergic neurodegeneration parameters. Interestingly, increased lethality (%) was found for all chemicals at the higher concentrations tested (100 and 500 mu M), with significant differences at 500 mu M DCA (p < 0.05). A decrease in the median survival was observed mainly for DCA. Although no changes were observed in RONS production, GSH levels were significantly increased upon diuron and DCA treatment, likely reflecting an attempt to restore the redox status. Moreover, diuron and its metabolites impaired ATP levels, suggesting an alteration in mitochondrial function. The latter may trigger autophagy as an adaptive survival mechanism, but this was not observed in C. elegans. Dopaminergic neurotoxicity was observed upon treatment with all the tested chemicals, but only diuron induced alterations in the worms' locomotor behavior. Combined, these results indicate that exposure to high concentrations of diuron and its metabolites elicit distinct adverse outcomes in C. elegans, and DCA in particular, plays an important role in the overall toxicity observed in this experimental model.
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