Fiber photometry in striatum reflects primarily nonsomatic changes in calcium

Fiber photometry enables recording of population neuronal calcium dynamics in awake mice. While the popularity of fiber photometry has grown in recent years, it remains unclear whether photometry reflects changes in action potential firing (that is, 'spiking') or other changes in neuronal calcium. In microscope-based calcium imaging, optical and analytical approaches can help differentiate somatic from neuropil calcium. However, these approaches cannot be readily applied to fiber photometry. As such, it remains unclear whether the fiber photometry signal reflects changes in somatic calcium, changes in nonsomatic calcium or a combination of the two. Here, using simultaneous in vivo extracellular electrophysiology and fiber photometry, along with in vivo endoscopic one-photon and two-photon calcium imaging, we determined that the striatal fiber photometry does not reflect spiking-related changes in calcium and instead primarily reflects nonsomatic changes in calcium. Fiber photometry can record brain dynamics, but the biological source of the signal remains unclear. The authors report that fiber photometry in striatum mainly reflects nonsomatic, and not somatic or spiking-related, changes in calcium.

Automated summary

Fiber photometry can record neuronal calcium dynamics in awake mice, but the biological source of the signal is still unclear. Through various experiments, the authors found that fiber photometry signal in the striatum primarily reflects nonsomatic changes in calcium.