期刊
NATURE METHODS
卷 19, 期 11, 页码 1490-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41592-022-01650-9
关键词
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资金
- NIH/NCI [P50 CA62924]
- NIH/NIDDK [K08 DK107781]
- Sol Goldman Pancreatic Cancer Research Center
- Buffone Family Gastrointestinal Cancer Research Fund
- Carol S. and Robert M. Long Pancreatic Cancer Research Fund
- Allegheny Health Network
- Johns Hopkins Cancer Research Fund
- American Cancer Society
- Cornelia T. Bailey Foundation [RSG-18-143-01]
- AACR-Bristol-Myers Squibb Midcareer Female Investigator Grant
- Emerson Collective Cancer Research Fund
- Robert L. Fine Pancreatic Cancer Research Foundation
- Rolfe Pancreatic Cancer Foundation
- Gerald O Mann Charitable Foundation
- SKCCC Cancer Center Support grant (CCSG) [P30 CA006973]
- National Institutes of Health/National Cancer Institute [UG3CA275681, U54CA268083, U54CA210173]
- National Institutes of Health/National Institute on Aging [U01AG060903]
- Rolfe Foundation for Pancreatic Cancer Research, Allegheny Health Network-Johns Hopkins Cancer Research Fund
- ARCS Foundation, Inc.
- NVIDIA GPU grant
- Carl and Carol Nale Fund for Pancreatic Cancer Research
- Nanotechnology for Cancer Research T32 Training grant [5T32CA153952]
- Cancer Research Fund
This study introduces a method called CODA for reconstructing three-dimensional microanatomical structures in large tissues using serially sectioned stained sections. The researchers demonstrated the effectiveness of CODA in reconstructing structures in pancreas, skin, lung, and liver tissues. They also applied CODA to analyze the microanatomy of the human pancreas during tumorigenesis.
A central challenge in biology is obtaining high-content, high-resolution information while analyzing tissue samples at volumes relevant to disease progression. We address this here with CODA, a method to reconstruct exceptionally large (up to multicentimeter cubed) tissues at subcellular resolution using serially sectioned hematoxylin and eosin-stained tissue sections. Here we demonstrate CODA's ability to reconstruct three-dimensional (3D) distinct microanatomical structures in pancreas, skin, lung and liver tissues. CODA allows creation of readily quantifiable tissue volumes amenable to biological research. As a testbed, we assess the microanatomy of the human pancreas during tumorigenesis within the branching pancreatic ductal system, labeling ten distinct structures to examine heterogeneity and structural transformation during neoplastic progression. We show that pancreatic precancerous lesions develop into distinct 3D morphological phenotypes and that pancreatic cancer tends to spread far from the bulk tumor along collagen fibers that are highly aligned to the 3D curves of ductal, lobular, vascular and neural structures. Thus, CODA establishes a means to transform broadly the structural study of human diseases through exploration of exhaustively labeled 3D microarchitecture. CODA: a method for 3D reconstruction of large serially sectioned tissues.
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