期刊
NANO LETTERS
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.2c02560
关键词
biomimetic nanoparticles; pDNA intracellular delivery; gene therapy; primary bone marrow mesenchymal macrophage membrane; multidrug-resistant bacterial sepsis
类别
资金
- National Natural Science Foundation of China [81971731, 81971733]
- Science Foundation for Distinguished Young Scholars of Tianjin [19JCJQJC62200]
- CAMS Innovation Fund for Medical Sciences (CIFMS) [2021-I2M-1-042, 2021-I2M-1-058]
- Nonprofit Central Research Institute Fund of Chinese Academy of Medical Sciences [2018PT35031]
Sepsis is a life-threatening disease with high morbidity and mortality. Antibiotic therapy is challenged by impaired macrophages and drug-resistant bacteria. In this study, a membrane-camouflaged metal-organic framework system was developed for efficient delivery of plasmid DNA to combat sepsis, significantly improving the survival rate of septic mice.
Sepsis is a life-threatening disease caused by systemic bacterial infections, with high morbidity and mortality worldwide. As the standard treatment for sepsis, antibiotic therapy faces the challenge of impaired macrophages and drug-resistant bacteria. In this study, we developed a membrane-camouflaged metal-organic framework (MOF) system for plasmid DNA (pDNA) delivery to combat sepsis. The antimicrobial gene LL37 was efficiently encapsulated in the pH-sensitive MOF, and the nanoparticles were decorated with macrophage membranes in a compatible manner. Macrophage membrane coating allows targeted delivery of LL37 to macrophages and creates macrophage factories for the continuous generation of antimicrobial peptides. Compared to naked nanoparticles, primary bone marrow mesenchymal macrophage membrane-modified nanoparticles greatly improved the survival rate of immunodeficient septic mice through the synergistic effect of efficient gene therapy and inflammatory cytokine sequestration. This study demonstrates an effective membrane biomimetic strategy for efficiently delivering pDNA, offering an excellent option for overcoming sepsis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据