期刊
NANO LETTERS
卷 22, 期 17, 页码 7173-7179出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.2c02447
关键词
DNA origami; self-assembly; folding pathways; seam; AFM
类别
资金
- National Key Research and Development Program of China [2021YFA1200900]
- National Natural Science Foundation of China [12174407, 12074208, 21934007, 21991134]
- Shanghai Municipal Science and Technology Commission Project [19JC1410300]
- Open Large Infra- structure Research of Chinese Academy of Sciences
In this study, the folding processes of several multidomain DNA origami structures were visualized using atomic force microscopy under ambient annealing conditions in solution, revealing the coexistence of diverse transitional structures that might result in the same prescribed products. Based on experimental observations and energy landscape simulations, the heterogeneity of the folding pathways of multidomain DNA origami structures was proposed.
A myriad of DNA origami nanostructures have been demonstrated in various intriguing applications. In pursuit of facile yet high-yield synthesis, the mechanisms underlying DNA origami folding need to be resolved. Here, we visualize the folding processes of several multidomain DNA origami structures under ambient annealing conditions in solution using atomic force microscopy with submolecular resolution. We reveal the coexistence of diverse transitional structures that might result in the same prescribed products. Based on the experimental observations and the simulation of the energy landscapes, we propose the heterogeneity of the folding pathways of multidomain DNA origami structures. Our findings may contribute to understanding the high-yield folding mechanism of DNA origami.
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