期刊
MYCORRHIZA
卷 32, 期 5-6, 页码 361-371出版社
SPRINGER
DOI: 10.1007/s00572-022-01091-4
关键词
AM fungi; Single spore; Morphology; Phylogeny; rDNA; Single nucleus sequencing
资金
- Uppsala University - European Research Council [678792]
- European Research Council (ERC) [678792] Funding Source: European Research Council (ERC)
In this study, genomes of AM fungal spores sampled from the field were successfully sequenced and assembled, demonstrating the feasibility of including uncultured AM fungi in genomic analysis. This opens up the possibility to study genomic variation in natural populations of these important plant symbionts.
Arbuscular mycorrhizal (AM) fungi are ubiquitous mutualistic symbionts of most terrestrial plants and many complete their lifecycles underground. Whole genome analysis of AM fungi has long been restricted to species and strains that can be maintained under controlled conditions that facilitate collection of biological samples. There is some evidence suggesting that AM fungi can adapt to culture resulting in phenotypic and possibly also genotypic changes in the fungi. In this study, we used field isolated spores of AM fungi and identified them as Funneliformis geosporum based on morphology and phylogenetic analyses. We separately assembled the genomes of two representative spores using DNA sequences of 19 and 22 individually amplified nuclei. The genomes were compared with previously published data from other members of Glomeraceae including two strains of F. mosseae. No significant differences were observed among the species in terms of gene content, while the single nucleotide polymorphism density was higher in the strains of F. geosporum than in the strains of F. mosseae. In this study, we demonstrate that it is possible to sequence and assemble genomes from AM fungal spores sampled in the field, which opens up the possibility to include uncultured AM fungi in phylogenomic and comparative genomic analysis and to study genomic variation in natural populations of these important plant symbionts.
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