4.6 Article

A Rapid and Sensitive LC-MS/MS Method for the Quantitation of Physalin A with Special Consideration to Chemical Stability in Rat Plasma: Application to a Pharmacokinetic Study

期刊

MOLECULES
卷 27, 期 21, 页码 -

出版社

MDPI
DOI: 10.3390/molecules27217272

关键词

physalin A; LC-MS; MS; stability; pharmacokinetics

资金

  1. National Natural Science Foundation of China [81873079]
  2. Technology Major Project of China Key New Drug Creation and Manufacturing Program [2017ZX09301012-001]

向作者/读者索取更多资源

Physalin A, a natural product with promising anti-inflammatory and anti-tumor activities, was analyzed in rat plasma using a stable LC-MS/MS method. The developed method demonstrated good linearity and was successfully applied to pharmacokinetic studies of Physalin A in rats, providing valuable insights for future research on this compound.
Physalin A is a promising natural product with excellent anti-inflammatory and anti-tumor activities. However, the pharmacokinetic profile of physalin A is still unclear. In this study, a rapid and sensitive analytical method based on LC-MS/MS for the quantitation of physalin A in rat plasma with special consideration to its chemical stability was developed and validated. To avoid the degradation of physalin A, the separation of plasma was conducted at 4 degrees C directly after the blood samples were collected. Meanwhile, plasma samples were immediately precipitated with acetonitrile containing tolbutamide (internal standard, IS) and the pH of the supernatant was adjusted to 1.5 with formic acid. Chromatographic separation of physalin A and IS was achieved on an ACQUITY UPLC BEH-C18 column (2.1 x 50 mm, 1.7 mu m) using 0.1% formic acid and acetonitrile as mobile phase delivered at 0.3 mL/min in a gradient elution mode. Physalin A and IS were detected through negative ion electrospray ionization in multiple reaction monitoring (MRM) mode. The MS/MS ion transitions for physalin A and IS were m/z 525.1-148.9 and m/z 269.8-169.9, respectively. The developed method showed good linearity over the range of 2.00-400 ng/mL. This method was successfully applied to the pharmacokinetic study of physalin A in rats following its intragastric administration and the findings were beneficial for future studies of physalin A.

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