期刊
MOLECULES
卷 27, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/molecules27186115
关键词
viral myocarditis; dilated cardiomyopathy; metabolomics; NMR; cardiac tissue
资金
- National Natural Science Foundation of China [31971357, 81160032, 21906147, 32271496]
- Natural Science Foundation of Guangxi Province [2018JJB140169]
- Joint Funds for the Innovation of Science Technology of Fujian Province [2018Y9100]
In this study, metabolomic analysis of cardiac tissues was conducted to investigate the metabolic changes in different pathological stages of viral myocarditis. The results revealed distinct metabolic differences between the pathological groups and normal controls, and identified impaired metabolic pathways and potential cardiac biomarkers associated with these pathological stages.
Viral myocarditis (VMC), which is defined as inflammation of the myocardium with consequent myocardial injury, may develop chronic disease eventually leading to dilated cardiomyopathy (DCM). Molecular mechanisms underlying the progression from acute VMC (aVMC), to chronic VMC (cVMC) and finally to DCM, are still unclear. Here, we established mouse models of VMC and DCM with Coxsackievirus B3 infection and conducted NMR-based metabolomic analysis of aqueous metabolites extracted from cardiac tissues of three histologically classified groups including aVMC, cVMC and DCM. We showed that these three pathological groups were metabolically distinct from their normal counterparts and identified three impaired metabolic pathways shared by these pathological groups relative to normal controls, including nicotinate and nicotinamide metabolism; alanine, aspartate and glutamate metabolism; and D-glutamine and D-glutamate metabolism. We also identified two extra impaired metabolic pathways in the aVMC group, including glycine, serine and threonine metabolism; and taurine and hypotaurine metabolism Furthermore, we identified potential cardiac biomarkers for metabolically distinguishing these three pathological stages from normal controls. Our results indicate that the metabolomic analysis of cardiac tissues can provide valuable insights into the molecular mechanisms underlying the progression from acute VMC to DCM.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据