4.6 Article

Synthesis of Phenylboronic Acid-Functionalized Magnetic Nanoparticles for Sensitive Soil Enzyme Assays

期刊

MOLECULES
卷 27, 期 20, 页码 -

出版社

MDPI
DOI: 10.3390/molecules27206883

关键词

magnetic nanoparticle; soil enzyme; protein capture; magnetic enrichment

资金

  1. Natural Foundation of Ningxia Hui Nationality Autonomous Region [2021BEG02002]
  2. Natural Science Foundation of Tianjin [21YFSNSN00030]
  3. Tianjin Agricultural Science and Technology Achievement Conversion and Extension Project [202101100, 201901130, 201901030]

向作者/读者索取更多资源

This study synthesized functionalized magnetic nanoparticles for efficiently capturing soil enzymes and sensitively assessing enzyme activity. The functionalized nanoparticles showed higher protein-capturing capacity and effectively captured different types of soil enzymes. Based on these nanoparticles, a method for determining trace enzyme activity in rhizosphere soil was developed.
Soil enzymes, such as invertase, urease, acidic phosphatase and catalase, play critical roles in soil biochemical reactions and are involved in soil fertility. However, it remains a great challenge to efficiently concentrate soil enzymes and sensitively assess enzyme activity. In this study, we synthesized phenylboronic acid-functionalized magnetic nanoparticles to rapidly capture soil enzymes for sensitive soil enzyme assays. The iron oxide magnetic nanoparticles (MNPs) were firstly prepared by the co-precipitation method and then functionalized by (3-aminopropyl)triethoxysilane, polyethyleneimine and phenylboric acid in turn, obtaining the final nanoparticles (MNPPBA). Protein-capturing assays showed that the functionalized MNPs had a much higher protein-capturing capacity than the naked MNPs (56% versus 6%). Moreover, MNPPBA almost thoroughly captured the tested enzymes, i.e., urease, invertase, and alkaline phosphatase, from enzyme solutions. Based on MNPPBA, a soil enzyme assay method was developed by integration of enzyme capture, magnetic separation and trace enzyme analysis. The method was successfully applied in determining trace enzyme activity in rhizosphere soil. This study provides a strategy to sensitively determine soil enzyme activity for mechanistic investigation of soil fertility and plant-microbiome interaction.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据