期刊
MOLECULES
卷 27, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/molecules27196719
关键词
Picea abies bark; green extraction; supercritical CO2; ultrasound assisted extraction; microwave assisted extraction; antioxidant; enzyme inhibition assays
资金
- Department of Pharmaceutical and Pharmacological Sciences (University of Padova) [PRID 2020]
This study extracted secondary metabolites from sawmill waste Picea abies bark using an innovative two-step extraction method. Different extraction techniques were compared for yield and compound concentration. The extracts were evaluated for potential pharmaceutical, nutraceutical, and cosmetic uses, as well as their antioxidant and enzyme inhibitory activities. Results showed that the smart extraction chain had advantages in terms of extraction yield and phytoconstituent concentration.
Secondary metabolites from the sawmill waste Picea abies bark were extracted using an innovative two-step extraction that includes a first step with supercritical CO2 (SCO2) and a second step using green solvents, namely ethanol, water, and water ethanol mixture. Maceration (M), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE) techniques were applied in the second step. A total of nineteen extract were obtained and yield were compared. Bark extracts were characterized by LC-DAD-MSn and classes of compounds were quantified as abietane derivatives, piceasides, flavonoids, and phenolics to compare different extractions. Obtained extracts were studied by in vitro assay to evaluate potential pharmaceutical, nutraceutical and cosmetic uses assessing the antioxidant activity as well as the inhibitory activity on target enzymes. Results show that the smart extraction chain is advantageous in term of yield of extraction and phytoconstituent concentration. SCO2 extract, presenting a unique composition with a large amount of abietane derivatives, exerted the best activity for amylase inhibition compared to the other extracts.
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