期刊
MOLECULES
卷 27, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/molecules27185848
关键词
antibacterial agents; synthetic cationic antimicrobial peptides; medicinal leech; cell membrane disruption; plasma coagulation
资金
- Russian Science Foundation [20-15-00270]
This study evaluated the behavior of synthetic short CAMPs in direct contact with blood cells and plasma. The results showed a correlation between CAMP biocompatibility and their effects on blood cell membranes and plasma.
Cationic antimicrobial peptides (CAMPs) are considered as next-generation antibiotics with a lower probability of developing bacterial resistance. In view of potential clinical use, studies on CAMP biocompatibility are important. This work aimed to evaluate the behavior of synthetic short CAMPs (designed using bioinformatic analysis of the medicinal leech genome and microbiome) in direct contact with blood cells and plasma. Eight CAMPs were included in the study. Hemolysis and lactate dehydrogenase assays showed that the potency to disrupt erythrocyte, neutrophil and mononuclear cell membranes descended in the order pept_1 > pept_3 similar to pept_5 > pept_2 similar to pept_4. Pept_3 caused both cell lysis and aggregation. Blood plasma and albumin inhibited the CAMP-induced hemolysis. The chemiluminescence method allowed the detection of pept_3-mediated neutrophil activation. In plasma coagulation assays, pept_3 prolonged the activated partial thromboplastin time (APTT) and prothrombin time (at 50 mu M by 75% and 320%, respectively). Pept_3 was also capable of causing fibrinogen aggregation. Pept_6 prolonged APTT (at 50 mu M by 115%). Pept_2 was found to combine higher bactericidal activity with lower effects on cells and coagulation. Our data emphasize the necessity of investigating CAMP interaction with plasma.
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