期刊
MOLECULAR NEUROBIOLOGY
卷 59, 期 11, 页码 7025-7035出版社
SPRINGER
DOI: 10.1007/s12035-022-03019-7
关键词
A1 adenosine receptor; P2X3 receptor; Electrophysiology; Dorsal root ganglion neuron; Nociceptive behavior
资金
- National Natural Science Foundation of China [81671101]
The results of this study indicate that the A1 adenosine receptor agonist CPA can inhibit the activity of P2X3 receptors in rat sensory neurons by activating A1 adenosine receptors and its downstream cAMP signaling pathway, revealing a novel peripheral mechanism underlying its analgesic effect.
Purinergic signaling is involved in multiple pain processes. P2X3 receptor is a key target in pain therapeutics, while A1 adenosine receptor signaling plays a role in analgesia. However, it remains unclear whether there is a link between them in pain. The present results showed that the A1 adenosine receptor agonist N-6-cyclopentyladenosine (CPA) concentration dependently suppressed P2X3 receptor-mediated and alpha,beta-methylene-ATP (alpha,beta-meATP)-evoked inward currents in rat dorsal root ganglion (DRG) neurons. CPA significantly decreased the maximal current response to alpha,beta-meATP, as shown a downward shift of the concentration-response curve for alpha,beta-meATP. CPA suppressed ATP currents in a voltage-independent manner. Inhibition of ATP currents by CPA was completely prevented by the A1 adenosine receptor antagonist KW-3902, and disappeared after the intracellular dialysis of either the G(i/o) protein inhibitor pertussis toxin, the adenylate cyclase activator forskolin, or the cAMP analog 8-Br-cAMP. Moreover, CPA suppressed the membrane potential depolarization and action potential bursts, which were induced by alpha,beta-meATP in DRG neurons. Finally, CPA relieved alpha,beta-meATP-induced nociceptive behaviors in rats by activating peripheral A1 adenosine receptors. These results indicated that CPA inhibited the activity of P2X3 receptors in rat primary sensory neurons by activating A1 adenosine receptors and its downstream cAMP signaling pathway, revealing a novel peripheral mechanism underlying its analgesic effect.
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