BCyto: A shiny app for flow cytometry data analysis

Flow cytometry (FCM) is a widely used technique to simultaneously measure various characteristics of a cell or particle. Most of reliable software for FCM data analysis are commercially available at high costs. A few R packages are available; however, programming skills are required. In addition, currently, no package offers an interactive user interface (UI) with efficient tools for conventional FCM data analysis. BCyto is an open-source R package/shiny app that allows cell biologists with no coding skills to reliably analyse FCM data in R. BCyto provides intuitive axis transformation for better data visualization, easy visualization of compensation plots and modification of compensation matrices, fast generation of backgating and overlay plots, as well as built-in proliferation and dimensionality reduction tools. BCyto will not only improve accessibility to FCM data anal-ysis but might also serve as an environment for the development of further R-based computational FCM algorithms.

Automated summary

BCyto is an open-source R package/shiny app that allows cell biologists without coding skills to reliably analyze FCM data in R. It provides various useful tools and an intuitive interface for data visualization and analysis.