期刊
MICROCHEMICAL JOURNAL
卷 181, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2022.107845
关键词
IBPHF; Potential-resolved; Electrochemiluminescence; Differential immunosensor; AFB1
资金
- National Natural Science Foundation of China [21705084]
- Natural Science Foundation of Shandong Province of China [ZR2017BB074]
- National Training Program of Innovation and Entrepreneurship for Undergraduates [S202010431027]
- Qilu University of Technology of Training Program of Innovation and Entrepreneurship for Undergraduates [xj201910431125]
- Research Leader Workshop of Jinan [2021GXRC101]
- Key Research and Development Program of Shandong Province [2021CXGC010808]
- Innovation Team of Jinan City [2018GXRC004]
- Special Funds for Taishan Scholars Project
In this study, a novel organoboron complex microrod with potential-resolved electrochemiluminescence (PRECL) property was synthesized. A competitive potential-resolved differential electrochemiluminescence immunosensor was fabricated using the microrod as a carrier to label competing antigens. The sensor exhibited excellent sensitivity and accuracy and achieved detection of target substances in walnut samples.
Electrochemiluminophore system with potential-resolved has exhibited giant potentiality for the application of bioassays in recent years, however, rarely studies toward the novel luminophore with potential-resolved electrochemiluminescence (PRECL) have been reported. In this work, a novel organoboron complex microrod (IBPHF) with PRECL property was synthesized, which emitted two strongest electrochemiluminescence (ECL) signals in the anode region at around 1.5 V and cathode region at around-1.5 V by using tri-n-propylamine (TPA)/K(2)S(2)O(8 )as coreactant. The relevant ECL properties and luminescence mechanisms have been discussed in detail. Moreover, electroactive substrate of three-dimensional porous starch carbon nanomaterials (3D-PSC NPs) were designed to capture antibodies. As a result, the competitive potential-resolved differential electro-chemiluminescence immunosensor was fabricated, and greatly enhanced sensitivity was achieved by using IBPHF by using IBPHF as a carrier to label competing antigens. In the presence of aflatoxin B1 (AFB1), due to the competitive mechanism and the specific recognition of antigen-antibody, the well-prepared immunosensor performed a quantitative decreasement in the range from 50 fg/mL to 100 ng/mL with a minimum detectable limit of 16.44 fg/mL by using the intensity difference (oECL) (ECLanode-ECLcathode). The self-correction of oECL extremely enhanced the accuracy and reliability of the sensor. Furthermore, the designed sensor achieved excellent detection towards AFB1 in walnut samples, and performed a bright application for trace target detection in future.
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