Scalable synthesis of Ce-MOF derived CeO/C Hierarchical: Efficient electrochemical sensing of uric acid as potential biomarker in acute myeloid leukaemia patients

Uric acid is an end product of purine metabolic pathway. Any alteration in its metabolism leads to diseases including acute myeloid leukemia (AML). In AML patients, uric acid levels are the potential predictor of disease aggressiveness. Various detection techniques have been used for uric acid analysis from biological samples. Among these, electrochemical sensing is specific, rapid, automatic, and economical. An electrical impulse detects in the form of redox peaks obtained via cyclic voltammetry and differential pulse voltammetry. Herein, a Ce-MOF sensor is developed for uric acid determination through CV and DPV. Ce-MOF is synthesized in a single-step and calcined at 400 & DEG;C. Sensing parameters like pH, concentration, scan rate, and effect of interferences are opti-mized. Linearity, limit of detection (LOD), and limit of quantification (LOQ) obtained from CV and DPV are 0.9950 M, 0.26 x 10(- 3) M, and 8.7 x 10(- 3) M, and 0.966 x 10-6M, 0.9 x 10(-6)M, and 9.68 x 10(-6)M, respectively. The stability of Ce-MOF-400 ? modified electrode is measured by 100 CV cycles. Ce-MOF-400 ? quantitatively determines uric acid from serum samples of Acute Myeloid Leukemia (AML) patients. The normal distribution of biological parameters data is determined by Shapiro-Wilk analysis. Positive and negative correlations of uric acid with other biological parameters are observed by paired t-test.

Automated summary

Uric acid metabolism is associated with diseases such as acute myeloid leukemia. Electrochemical sensing is a specific, rapid, automatic, and economical method for uric acid analysis. This study developed a Ce-MOF sensor for uric acid determination, showing good linearity and detection limit.