期刊
MICROBIAL PATHOGENESIS
卷 170, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2022.105669
关键词
Carbapenem-resistant E. coli; Class 1 integrons; Integron variable region promoters; Gene cassettes
资金
- Health Commission of Weifang [wfwsjk-2020-035]
This study investigated the distribution of class 1 integrons and their variable regional molecular characteristics in CR-Eco strains, as well as the diversity of promoter types and drug sensitivity. The positive rate of class 1 integrons in CR-Eco strains was high, and these strains showed significant resistance to multiple antimicrobial drugs. The findings provide valuable insights into the mechanisms and transmission of drug resistance in these strains.
Objective: To investigate the distribution of class 1 integrons and their variable regional molecular characteristics, as well as the diversity of promoter and drug sensitivity of CR-Eco (carbapenem-resistant E. coli) strains. Method: A total of 117 CR-Eco strains, collected between 2012.01 and 2019.12, underwent fully automated bacterial identification and sensitization using VITEK-2 Compact and supplemented by K-B assay. PCR was employed to screen for class 1 integrase genes and integron variable regions, while the promoter type and variable region gene cassette characteristics were determined by sequencing analysis. Results: The positive rate of the class 1 integron of the CR-Eco strains was 83.70% (92/117) herein. Moreover, class 1 integrase-positive strains exhibited statistically significant resistance to aztreonam, ceftazidime, ciprofloxacin, ceftriaxone, gentamicin, meropenem, and trimethoprim-sulfamethoxazole compared to integron-negative strains (P < 0.05). Variable regions were observed in 77 of the 92 class 1 integrase-positive strains. In addition, seven gene cassettes were detected, namely dfrA17-aadA5, aadA22, dfrA12-aadA2, dfrA12, dfrA17, dfrA27 and aadA. Finally, five types of class 1 integron variable region promoters were identified in those 77 strains, including PcW, PcH1, PcWTGN-10, PcH1(TGN-10), and P2, which were detected in 48, 18, 8, 2, and 1 strains, respectively. Conclusion: The primary integrator variable region gene cassettes of this class were dfrA and aadA. The integron-positive strains displayed simultaneous high resistance to multiple antimicrobial drugs. The integrator variable region promoters of the CR-Eco strains are primarily weak and can potentially form and spread drug resistance.
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