4.7 Article

Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biotechnology & Applied Microbiology

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor-binding domain in engineered Komagataella phaffii

Neil C. Dalvie et al.

Summary: Genetically engineered Komagataella phaffii strain was developed to produce RBD protein without methanol induction, increasing production efficiency. This engineered strain enables scalability for manufacturing RBD-based vaccine antigens and other variants for future vaccines.

BIOTECHNOLOGY AND BIOENGINEERING (2022)

Article Multidisciplinary Sciences

A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses

Tiong Kit Tan et al.

Summary: This study introduces a potential vaccine candidate against SARS-CoV-2, utilizing a synthetic virus-like particle platform to present the receptor-binding domain of the virus spike protein. The vaccine induces strong neutralizing antibody responses in mice and pigs, demonstrating thermostability and the potential for global distribution.

NATURE COMMUNICATIONS (2021)

Article Biology

Comparison of Different Signal Peptides for the Efficient Secretion of the Sweet-Tasting Plant Protein Brazzein in Pichia pastoris

Fabrice Neiers et al.

Summary: Brazzein is a sweet-tasting protein found in the red berries of a West African shrub, with potential use as a natural sweetener. Heterologous expression of brazzein in yeast Pichia pastoris allows for efficient production of functional brazzein, with three signal peptides resulting in high levels of secretion.

LIFE-BASEL (2021)

Article Biotechnology & Applied Microbiology

Modifications in the Kex2 P1′ cleavage site in the α-MAT secretion signal lead to higher production of human granulocyte colony-stimulating factor in Pichia pastoris

Sakshi Aggarwal et al.

Summary: This study investigated the use of modified signal sequences in Pichia pastoris to enhance the production of G-CSF, shedding light on the role of conformational changes induced by the interaction between the signal sequence and the cargo protein. The results showed that replacing Glu at the P1' position of the Kex2 cleavage site with Val/Ala significantly increased the extracellular production of G-CSF, providing insights for improving the yield of therapeutic proteins in yeast hosts.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2021)

Article Biotechnology & Applied Microbiology

Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

Neil C. Dalvie et al.

Summary: This study demonstrates a holistic approach for molecular design of recombinant protein antigens, improving manufacturability and reducing product variants while maintaining original antigenicity and immunogenicity. The engineered NRRV antigens enabled simultaneous production and purification of all three serotypes in a single process, showcasing the potential to lower cost and volumetric requirements in manufacturing vaccines based on recombinant protein subunits.

MICROBIAL CELL FACTORIES (2021)

Article Multidisciplinary Sciences

Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice

Neil C. Dalvie et al.

Summary: Subunit vaccines based on recombinant proteins are crucial tools for controlling COVID-19 due to their low manufacturing cost, scalability, and proven safety and efficacy. Engineered RBD variants exhibit high manufacturability and immunogenicity, showing enhanced immune responses after a single dose and cross-reactivity to new variants of concern.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Biotechnology & Applied Microbiology

Comparative genome-scale analysis of Pichia pastoris variants informs selection of an optimal base strain

Joseph R. Brady et al.

BIOTECHNOLOGY AND BIOENGINEERING (2020)

Article Biochemical Research Methods

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

Neil C. Dalvie et al.

ACS SYNTHETIC BIOLOGY (2020)

Article Pharmacology & Pharmacy

Eptinezumab: First Approval

Sohita Dhillon

Article Biotechnology & Applied Microbiology

A combined screening and in silico strategy for the rapid design of integrated downstream processes for process and product-related impurity removal

Nicholas Vecchiarello et al.

BIOTECHNOLOGY AND BIOENGINEERING (2019)

Review Biochemical Research Methods

Continuous integrated manufacturing of therapeutic proteins

Daniel Johannes Karst et al.

CURRENT OPINION IN BIOTECHNOLOGY (2018)

Review Biochemical Research Methods

The yeast stands alone: the future of protein biologic production

Kerry R. Love et al.

CURRENT OPINION IN BIOTECHNOLOGY (2018)

Article Biotechnology & Applied Microbiology

On-demand manufacturing of clinical-quality biopharmaceuticals

Laura E. Crowell et al.

NATURE BIOTECHNOLOGY (2018)

Review Biotechnology & Applied Microbiology

Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications

Zhen Kang et al.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2017)

Review Biochemistry & Molecular Biology

Cell Factory Engineering

Anne Mathilde Davy et al.

CELL SYSTEMS (2017)

Review Biotechnology & Applied Microbiology

Reexamining opportunities for therapeutic protein production in eukaryotic microorganisms

Catherine B. Matthews et al.

BIOTECHNOLOGY AND BIOENGINEERING (2017)

Review Biotechnology & Applied Microbiology

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

Mudassar Ahmad et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris

Shuli Liang et al.

BIOTECHNOLOGY LETTERS (2013)

Article Biotechnology & Applied Microbiology

Enabling global access to high-quality biopharmaceuticals

J. Christopher Love et al.

CURRENT OPINION IN CHEMICAL ENGINEERING (2013)

Review Microbiology

The secretory pathway: exploring yeast diversity

Marizela Delic et al.

FEMS MICROBIOLOGY REVIEWS (2013)

Article Medicine, Research & Experimental

Synthesis and secretory expression of hybrid antimicrobial peptide CecA-mag and its mutants in Pichia pastoris

Xiuqing Wang et al.

EXPERIMENTAL BIOLOGY AND MEDICINE (2012)

Review Biotechnology & Applied Microbiology

Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements

Chung-Jr Huang et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2012)

Article Biochemical Research Methods

A REVIEW SIZE-EXCLUSION CHROMATOGRAPHY FOR THE ANALYSIS OF PROTEIN BIOTHERAPEUTICS AND THEIR AGGREGATES

Paula Hong et al.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2012)

Article Biotechnology & Applied Microbiology

Evaluation of Protein Engineering and Process Optimization Approaches to Enhance Antibody Drug Manufacturability

Greg P. Conley et al.

BIOTECHNOLOGY AND BIOENGINEERING (2011)

Article Biotechnology & Applied Microbiology

Optimization of a glycoengineered Pichia pastoris cultivation process for commercial antibody production

Jianxin Ye et al.

BIOTECHNOLOGY PROGRESS (2011)

Article Biotechnology & Applied Microbiology

Antibody fragments may be incorrectly processed in the yeast Pichia pastoris

D. G. Kozlov et al.

BIOTECHNOLOGY LETTERS (2008)

Editorial Material Biochemistry & Molecular Biology

Pichia power: India's biotech industry puts unconventional yeast to work

Chandra Shekhar

CHEMISTRY & BIOLOGY (2008)

Article Biochemical Research Methods

Secretory expression of interferon-alpha 2b in recombinant Pichia pastoris using three different secretion signals

Anand Ghosalkar et al.

PROTEIN EXPRESSION AND PURIFICATION (2008)

Article Biotechnology & Applied Microbiology

Expression, purification and characterization of human IFN-λ1 in Pichia pastoris

Yun Fei Xie et al.

JOURNAL OF BIOTECHNOLOGY (2007)

Article Biotechnology & Applied Microbiology

Modifications of therapeutic proteins: challenges and prospects

Nigel Jenkins

CYTOTECHNOLOGY (2007)