4.5 Article

Chronic exposure to methadone induces activated microglia and astrocyte and cell death in the cerebellum of adult male rats

期刊

METABOLIC BRAIN DISEASE
卷 38, 期 1, 页码 323-338

出版社

SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11011-022-01108-z

关键词

Apoptosis; Cerebellum; Methadone; Neuroinflammation

向作者/读者索取更多资源

This study found that methadone treatment causes cerebellar atrophy, glial cell activation, astrocyte proliferation, apoptosis, and neuro-inflammation. Methadone also affects motor coordination, locomotor performance, and neuromuscular activity.
Methadone is a centrally-acting synthetic opioid analgesic widely used in the methadone maintenance therapy (MMT) programs throughout the world. Considering its neurotoxic effects particularly on the cerebellum, this study aims to address the behavioral and histological alterations in the cerebellar cortex associated with methadone administration. Twenty-four adult male albino rats were randomized into two groups of control and methadone treatment. Methadone was subcutaneously administered (2.5-10 mg/kg) once a day for two consecutive weeks. The functional and structural changes in the cerebellum were compared to the control group. Our data revealed that treating rats with methadone not only induced cerebellar atrophy, but also prompted the actuation of microgliosis, astrogliosis, and apoptotic biomarkers. We further demonstrated that treating rats with methadone increased complexity of astrocyte processes and decreased complexity of microglia processes. Our result showed that methadone impaired motor coordination and locomotor performance and neuromuscular activity. Additionally, relative gene expression of TNF-alpha, caspase-3 and RIPK3 increased significantly due to methadone. Our findings suggest that methadone administration has a neurodegenerative effect on the cerebellar cortex via dysregulation of microgliosis, astrogliosis, apoptosis, and neuro-inflammation.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据