期刊
CARBOHYDRATE POLYMERS
卷 134, 期 -, 页码 66-73出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.07.070
关键词
Laminaria japonica; Polysaccharides; Immunostimulation; Macrophage; TLR4
资金
- National Natural Science Foundation of China [31271814, 21006019]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20130111110001]
- Fundamental Research Funds for the Central Universities [2014HFCH0011]
In this work, a novel water-soluble homogeneous polysaccharide (LJP-31) with a molecular mass of 2.24 x 10(6) Da was isolated and purified from Laminaria japonica using DEAE-cellulose and Sephacryl S500 chromatography. Results showed that LJP-31 mainly consists of arabinose, mannose, glucose and galactose in a molar ratio of 1.0:7.8:6.6:0.8. LJP-31 exhibited significant stimulation on macrophages and enhanced the production of NO, TNF-alpha, IL-1 beta, IL-6 and IL-10 as well as the up-regulation of their gene expressions. Western blot analysis suggested that LJP-31 has the positive effects on the translocation of NF-kappa B p65 from cytoplasm to nucleus and the phosphorylation of I kappa B alpha, ERK1/2, JNK1/2 and P38 in macrophages. Flow cytometric and confocal laser-scanning microscopy analysis indicated that toll-like receptor 4 (TLR4) was at least one of the recognition receptors of LJP-31 on the plasma membrane of macrophages. Taken together, LJP-31 may exert its immunostimulating potency via TLR4 activation of MAPK and NF-kappa B signaling pathways. (C) 2015 Elsevier Ltd. All rights reserved.
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