Magnesium-doped biphasic calcium phosphate nanoparticles with incorporation of silver: Synthesis, cytotoxic and antibacterial properties

The development of new calcium phosphate nanoparticles with excellent cytocompatibility and antibacterial properties is generating substantial interest in the biomedical field. In this regard, the present study demon-strated the synthesis of magnesium-doped biphasic calcium phosphate nanoparticles containing silver (AgMgB-NPs) via the employment of the chemical wet-precipitation method. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and ultraviolet-visible spectroscopy (UV-Vis) methods were used to confirm the successful synthesis of AgMgB-NPs. X-ray photoelectron spectroscopy (XPS) and Raman spectra indicated that Mg2+ was doped at the Ca2+ position. The excellent cytocompatibility of AgMgB-NPs was confirmed using the cell counting kit-8 (CCK-8) analysis which employed a culture of human bone marrow-derived mesenchymal stem cells (hBMSCs). Additionally, the antibacterial activity of AgMgB-NPs was evaluated using Gram-negative E. coli and Gram-positive S. aureus micro-organisms. The present study therefore developed novel calcium phosphate nanoparticles that were demonstrated to have the potential for biomedical applications.

Automated summary

This study successfully synthesized magnesium-doped biphasic calcium phosphate nanoparticles containing silver and confirmed their excellent cytocompatibility and antibacterial activity.