期刊
MARINE DRUGS
卷 20, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/md20090545
关键词
G; lemaneiformis; polysaccharide; degradation; free radicals; antioxidant; signaling pathway
资金
- Key Area Research and Development Program of Guangdong Province [2020B1111030004]
- National Key R&D Program of China [2019YFD0901905]
- China Agriculture Research System of MOF and MARA [CARS-50]
- Special Scientific Research Funds for Central Nonprofit Institutes, Chinese Academy of Fishery Sciences [2020TD69]
- Central Public-Interest Scientific Institution Basal Research Funds, South China Sea Fisheries Research Institute, CAFS [2021SD06]
- Postgraduate Education Innovation Project of Guangdong Ocean University [202243, 202242]
- Guangdong Innovation Team of Seafood Green Processing Technology [2019KCXTD011]
The aim of this research was to investigate the antioxidant activity and underlying mechanism of Gracilaria lemaneiformis polysaccharide degradation in HepG2 cells. The results showed that the degraded polysaccharide had a higher scavenging ability for free radicals. Additionally, the polysaccharide relieved oxidative stress in the cells by modulating the Nrf-2/Keap-1 signaling pathway.
The objective of this research was to investigate the antioxidant activity of Gracilaria lemaneiformis polysaccharide degradation and its underlying mechanism involved in the Nrf-2/Keap-1 signaling pathway in HepG2 cells with oxidative stress induced by H2O2. The result of the scavenging ability of free radicals showed that GLP-HV (polysaccharide degraded by H2O2-vitamin C (Vc)) performed a better scavenging ability than GLP (G. lemaneiformis polysaccharide). Moreover, the scavenging ability of polysaccharide to these free radicals from strong to weak was as follows: superoxide radical, ferric ion, ABTS(+), and DPPH radical, and their IC50 values were 3.56 +/- 0.0028, 4.97 +/- 0.18, 9.62 +/- 0.35, and 23.85 +/- 1.78 mg/mL, respectively. Furthermore, GLP-HV obviously relieved oxidative stress in HepG2 cells, which strengthened the activity of T-AOC, CAT, GSH-PX, and SOD, and diminished the intensity of MDA, intracellular ROS, and calcium ion based on the Nrf-2/Keap-1 signaling pathway. The PCR result revealed that polysaccharide upregulated the expression of the genes Nrf-2, HO-1, NQO-1, and ZO-1 and downregulated Keap-1. The correlation between chemical properties and antioxidant mechanism of GLP-HV was evaluated via a heat map. The results illustrated that reducing sugar and active groups presented a positive correlation, and molecular weight and viscosity exhibited a negative relation with antioxidant activity.
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