4.7 Article

Glycoproteomics reveal differences in site-specific N-glycosylation of whey proteins between donkey colostrum and mature milk

期刊

LWT-FOOD SCIENCE AND TECHNOLOGY
卷 169, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.lwt.2022.113920

关键词

Donkey milk; Colostrum; Mature milk; Whey N-Glycoprotein; Site -specific glycoproteomics

资金

  1. National Key R & D Program of China [2018YFC1604302]
  2. National Science and Technology Plan Project [2013BAD18B03]
  3. Shenyang Technological Innovation Project [Y17-0028]
  4. LiaoNing Revitalization Talents Project [XLYC1902083]

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This study used site-specific glycoproteomics to investigate the N-glycosylation modifications of donkey whey protein. The results showed significant changes in site-specific N-glycans of donkey whey protein during lactation, providing new insights into the biological function of donkey whey protein.
Donkey whey protein has high nutritional value, yet systematic characterisation of site-specific N-glycosylation of donkey whey protein is still in its infancy. This study used label-free site-specific glycoproteomics to identify and quantify 685 intact N-glycopeptides from 135 whey glycoproteins in donkey colostrum (DC) and 384 intact N-glycopeptides from 67 glycoproteins in donkey mature milk (DM). As lactation progressed, 23 site-specific N-glycans mapping to 13 glycosites on 12 glycoproteins were significantly decreased, and 19 site-specific N-glycans mapping to 7 glycosites on 6 glycoproteins were significantly increased. We also found that certain N-glycans attached to the glycosites exhibited significant site specificity. This study provides new insights into the potential relationship between the biological function of donkey whey proteins and their N-glycosylation modifications. Furthermore, the study provides molecular evidence for differences in the site-specific N-glycosylation profile of donkey whey protein during lactation.

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