期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 169, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2022.113950
关键词
Acetic acid bacteria (AAB); Ethanol resistance; Membrane; Cytomembrane fluidity; Metabolism
资金
- Hubei 2019 Grain Circulation Devel- opment Industry Project
- Teaching Research Programs of Hubei Province
- Major Technical Innovation Project of Hubei Province
- [2020140823]
- [2018323]
- [2019ABA092]
This study investigated the mechanism of ethanol resistance in acetic acid bacteria and found that membrane stability and energy metabolism play important roles in ethanol tolerance.
Acetic acid bacteria (AAB) are widely used in acetic acid fermentation where the combined action of ethanol and acetic acid disturbs the cell viability. The present study investigated the ethanol resistance mechanisms through the comparisons between a more ethanol-tolerant Acetobacter pasteurianus JZ1601 and a less ethanol-tolerant A. pasteurianus 1.41 by focusing on the early stage of fermentation without acetic acid interference. While the membrane was the primary injury target, optimum membrane stability rather than increased membrane fluidity was demonstrated to be relevant to the ethanol tolerance. The weak membrane permeability entrusted JZ1601 with relatively reduced sublethal injury, low intracellular ethanol, and superior growth throughout the high ethanol challenge. However, the level of fatty acids could not play a direct role in improving the membrane stability. Furthermore, the tricarboxylic acid cycle, pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) and acetaldehyde dehydrogenase (PQQ-ALDH) activities were the potential to adapt to high ethanol challenges, with PQQ-ADH activity contributing more to the ethanol tolerance of JZ1601. These results demonstrated that the characteristic membrane structure, energy metabolism, and their improved adaptive regulation contributed to a high ethanol tolerance of AAB.
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