期刊
CARBOHYDRATE POLYMERS
卷 129, 期 -, 页码 25-34出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.04.036
关键词
All-trans-retinoic acid; Chitooligosaccharide; Co-delivery; Paclitaxel; Polymeric nanoparticles
资金
- Shandong Provincial Natural Science Foundation, China [ZR2013EMQ008, ZR2014HM096]
- Scientific Research Foundation from Binzhou Medical University [BY2010KYQD11]
- Shandong Province Higher Educational Science and Technology Program [J11LF88]
An amphiphilic all-trans-retinoic acid (ATRA)-chitooligosaccharide (RCOS) conjugate was synthesized to form self-assembled polymeric nanoparticles to facilitate the co-delivery of ATRA and paclitaxel (PTX). The blank RCOS nanoparticles possessed low hemolytic activity and cytotoxicity, and could efficiently load PTX with a drug loading of 22.2% and a high encapsulation efficiency of 71.3%. PTX-loaded RCOS nanoparticles displayed a higher cytotoxicity to HepG2 cells compared to PTX plus ATRA solution when corrected by the accumulated drug release. Cellular uptake profiles of RCOS nanoparticles were evaluated via confocal laser scanning microscope and flow cytometry with FITC as a fluorescent mark. The RCOS nanoparticles could be rapidly and continuously taken up by HepG2 cells via endocytosis and transported into the nucleus, and the uptake rates increased with particle concentration. These results revealed the promising potential of RCOS nanoparticles as drug carriers for co-delivery of ATRA and PTX or other hydrophobic therapeutic agents. (C) 2015 Elsevier Ltd. All rights reserved.
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