Photochemical Identification of Auxiliary Severe Acute Respiratory Syndrome Coronavirus 2 Host Entry Factors Using μMap

Article Multidisciplinary Sciences

SARS-CoV-2 Omicron variant replication in human bronchus and lung ex vivo

Kenrie P. Y. Hui et al.

Summary: SARS-CoV-2 variants pose a threat to global public health. The Omicron variant replicates faster in bronchi but less efficiently in the lung parenchyma compared to other variants. All variants of concern have similar cellular tropism.

NATURE (2022)

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Article Multidisciplinary Sciences

Striking antibody evasion manifested by the Omicron variant of SARS-CoV-2

Lihong Liu et al.

Summary: The B.1.1.529/Omicron variant of SARS-CoV-2, initially detected in southern Africa, has rapidly spread globally and is expected to become dominant due to its enhanced transmissibility in the coming weeks. This variant poses a threat to the efficacy of current COVID-19 vaccines and antibody therapies due to its significant antibody resistance. Even individuals who have received vaccines and booster doses may have reduced neutralizing activity against B.1.1.529.

NATURE (2022)

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Article Chemistry, Multidisciplinary

μMap-Red: Proximity Labeling by Red Light Photocatalysis

Benito F. Buksh et al.

Summary: This study reports a proximity labeling platform called mu Map-Red, which utilizes a red-light-excited catalyst to activate a biotin probe for labeling biomolecules. The platform has been validated for protein labeling in complex biological environments and animal models, and successfully applied to profile erythrocyte cell-surface proteins in whole mouse blood samples.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022)

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Article Cell Biology

Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant

Yang Liu et al.

Summary: This study reports that the P681R mutation in the Delta spike plays a crucial role in the replacement of the Alpha variant by the Delta variant during the COVID-19 pandemic. The Delta variant outcompetes the Alpha variant in human lung cells and airway tissues. The P681R mutation enhances the cleavage of the spike protein, leading to increased replication of the Delta variant.

CELL REPORTS (2022)

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Article Chemistry, Multidisciplinary

Spatially resolved cell tagging and surfaceome labeling via targeted photocatalytic decaging

Ziqi Liu et al.

Summary: In this study, we report a extracellular-targeted photocatalytic decaging system for spatially resolved cell tagging and surface proteome profiling. By conjugating an antibody to the photocatalysis system, we achieved targeted photocatalytic decaging on cell surfaces, allowing selective cell tagging in cell mixture and tumor xenografts. Furthermore, we combined quinone methide decaging chemistry with the photocatalytic system to achieve spatially resolved membrane proteome profiling, revealing potential protein clusters. Finally, we expanded our strategy to photocatalytic prodrug decaging for selective tumor cell killing.

CHEM (2022)

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Letter Biochemistry & Molecular Biology

SARS-CoV-2 Omicron RBD shows weaker binding affinity than the currently dominant Delta variant to human ACE2

Leyun Wu et al.

SIGNAL TRANSDUCTION AND TARGETED THERAPY (2022)

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Review Biochemical Research Methods

Deciphering molecular interactions by proximity labeling

Wei Qin et al.

Summary: This article introduces the proximity labeling technology for studying and applying molecular interactions. By fusing baits with promiscuous enzymes, endogenous molecules interacting with them can be tagged and identified by mass spectrometry or nucleic acid sequencing. PL technology has been used to map protein-protein, protein-RNA, and protein-DNA interactions.

NATURE METHODS (2021)

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Article Chemistry, Multidisciplinary

Bioorthogonal Photocatalytic Decaging-Enabled Mitochondrial Proteomics

Zongyu Huang et al.

Summary: CAT-Prox is a novel method for spatiotemporally resolved mitochondrial proteome profiling in living cells, which combines photocatalytic decaging chemistry with proximity-based protein labeling, offering high efficiency and mitochondrial specificity.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

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Article Multidisciplinary Sciences

Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells

Jian Chen et al.

Summary: The study identified MYH9 as an important host factor for SARS-CoV-2 infection of human pulmonary cells, mediating viral entry through endocytosis and bypassing the TMPRSS2 and CatB/L pathways. Loss of MYH9 reduces authentic SARS-CoV-2 infection in ACE2-expressing cells, suggesting it as a potential target for clinical intervention strategies in the future.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

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Article Genetics & Heredity

Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2

Jim Baggen et al.

Summary: Genome-wide CRISPR screens have revealed that the lysosomal protein TMEM106B is essential for SARS-CoV-2 infection. Overexpression of TMEM106B enhances both SARS-CoV-2 and pseudovirus infection, indicating a potential role in viral entry. Single-cell RNA-sequencing data from COVID-19 patient airway cells shows a correlation between TMEM106B expression and SARS-CoV-2 infection.

NATURE GENETICS (2021)

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Article Multidisciplinary Sciences

Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks

Maik Muller et al.

Summary: The spatial organization of cell surface receptors is critical for cell signaling and drug action. Here, the authors develop an optoproteomic method for mapping surface protein interactions, revealing cellular responses to antibodies, drugs and viral particles as well as immunosynapse signaling events.

NATURE COMMUNICATIONS (2021)

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Article Cell Biology

SARS-CoV-2 uses metabotropic glutamate receptor subtype 2 as an internalization factor to infect cells

Jinliang Wang et al.

Summary: mGluR2 has been identified as an internalization factor for SARS-CoV-2, facilitating viral entry into cells. It cooperates with ACE2 to promote virus internalization through clathrin-mediated endocytosis, and its knockout in mice significantly reduces viral infection in the nasal turbinates and lungs. mGluR2 is also important for the internalization of other coronaviruses, suggesting it may be a potential target for antiviral development.

CELL DISCOVERY (2021)

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Article Multidisciplinary Sciences

COVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets

Toni M. Delorey et al.

Summary: This study using single-cell and spatial atlases revealed the impact of COVID-19 infection on lung, kidney, liver, and heart tissues, showing structural remodeling and viral RNA enrichment. Differences in lung inflammatory responses and transcriptional alterations in heart tissue of COVID-19 patients were also identified, along with cell types and genes associated with disease severity.

NATURE (2021)

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Article Biochemistry & Molecular Biology

Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis

Zhen Chen et al.

Summary: The study identified 437 high-confidence interacting proteins between SARS-CoV-2 virus and host cells, providing valuable resources for understanding and treating COVID-19. Unique roles of different SARS-CoV-2 viral proteins in its life cycle were revealed, with potential drug targets discovered. Comparisons of interaction networks shed light on shared and divergent pathways manipulated by various human coronaviruses, possibly explaining differences in disease pathology.

EMBO JOURNAL (2021)

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Article Microbiology

DC/L-SIGN recognition of spike glycoprotein promotes SARS-CoV-2 trans-infection and can be inhibited by a glycomimetic antagonist

Michel Thepaut et al.

Summary: The study reveals that C-type lectin receptors (CLRS) can bind to the spike envelope protein of SARS-CoV-2, and DC-SIGN and L-SIGN among them can promote virus trans-infection. This discovery identifies a new mechanism for enhancing viral spread.

PLOS PATHOGENS (2021)

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Article Medicine, Research & Experimental

Nasal ciliated cells are primary targets for SARS-CoV-2 replication in the early stage of COVID-19

Ji Hoon Ahn et al.

Summary: Research shows that in the early stages of COVID-19, the virus replicates massively in multiciliated cells of the nasal epithelium, and infected ciliated cells are rapidly replaced by differentiating precursor cells.

JOURNAL OF CLINICAL INVESTIGATION (2021)

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Article Virology