期刊
CARBOHYDRATE POLYMERS
卷 129, 期 -, 页码 101-107出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.04.057
关键词
Sulfated polysaccharides; Fucoidan; Microwave assisted extraction; Ascophyllum nodosum; Antioxidant activity
Sulfated polysaccharides (fucoidan) from brown seaweed Ascophyllum nodosum were extracted by microwave assisted extraction (MAE) technology. Different conditions of temperature (90-150 degrees C), extraction time (5-30 min) were evaluated and optimal fucoidan yield was 16.08%, obtained from 120 degrees C for 15 min's extraction. Compositional analysis, GPC, HPAEC and IR analysis were employed for characterization of extracted sulfated polysaccharides. Fucose was the main monosaccharide of fucoidan extracted at 90 degrees C while glucuronic acid was the main monosaccharide of fucoidan extracted at 150 degrees C. Both the molecular weight and sulfate content of extracted fucoidan increased with decreasing extraction temperature. All fucoidans exhibited antioxidant activities as measured by DPPH scavenging and reducing power, among which fucoidan extracted at 90 degrees C was highest. This study shows that MAE is an efficient technology to extract sulfated polysaccharides from seaweed and Ascophyllum nodosum could potentially be a resource for natural antioxidants. (C) 2015 Elsevier Ltd. All rights reserved.
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