期刊
CARBOHYDRATE POLYMERS
卷 127, 期 -, 页码 38-46出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.02.043
关键词
Acanthopanax koreanum Nakai; Polysaccharide; Enzyme-assistant extract; Antioxidant activity; Zebrafish; Oxidative stress
资金
- Ministry of Trade, Industry and Energy (MOTIE)
- Korea Institute for Advancement of Technology (KIAT)
- Jeju Institute for Regional Program Evaluation through the Leading Industry Development for Economic Region
In this study, the antioxidative effects of a purified polysaccharide isolated from the stems of Acanthopanax koreanum Nakai (ASP) on hydrogen peroxide-induced oxidative stress was investigated both in vitro and in vivo using a zebrafish model. A. koreanum Nakai stem was hydrolyzed using five carbohydrases and five proteases for the enzyme-assistant extraction. Of the enzyme-assistant extracts, the Protamex extract exhibited the highest yield and a potent scavenging activity against free radicals, Ethanol-added separation and anion exchange chromatography were conducted to identify the active polysaccharide. The purified polysaccharide significantly scavenged hydrogen peroxide and reduced hydrogen peroxide-induced cell death in Vero cells and in zebrafish. The results reveal that ASP is a useful antioxidant polysaccharide and might be available for relevant industrial applications. (C) 2015 Elsevier Ltd. All rights reserved.
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