4.5 Article

Comprehensive analysis of dihydromyricetin metabolites in rats using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry

期刊

JOURNAL OF SEPARATION SCIENCE
卷 45, 期 21, 页码 3930-3941

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202200319

关键词

dihydromyricetin; metabolic profile; ultra-high-performance liquid chromatography; Q-Exactive Orbitrap mass spectrometry

资金

  1. General Program of the National Natural Science Foundation of China [82174039]
  2. General project of Shandong Natural Science Foundation [ZR2020MH371]
  3. Shandong Taishan Scholars Young Expert Project [tsqn202103110]
  4. Shandong Province Youth Talents Introducing and Cultivating Program [10073004]
  5. Key technologies for quality identification and control of Traditional Chinese Medicine Formula Granules [2021CXGC010511]

向作者/读者索取更多资源

This study established a rapid profiling and identification method for dihydromyricetin metabolites in rats and revealed the biotransformation pathways. A total of 49 metabolites were identified, providing a foundation for understanding the action mechanism of dihydromyricetin and other compounds in traditional Chinese medicines or natural plants.
As the most abundant and bioactive constituent in vine tea (Ampelopsis grossedentata), dihydromyricetin possesses numerous biological activities. A rapid profiling and identification method for dihydromyricetin metabolites in rats after the oral administration has been established using ultra-high-performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry coupled with multiple data-mining methods. Herein, an efficient analytical strategy characterized by a parallel reaction monitoring mode combining diagnostic fragment ions filtering techniques was developed for the comprehensive identification of dihydromyricetin metabolites in rat plasma, urine, and feces. And then, the biotransformation pathways of dihydromyricetin were further revealed. As a result, a total of 49 metabolites were finally identified by comparing diagnostic fragment ions, chromatographic retention times, neutral loss fragment ions, and accurate mass measurement with those of the dihydromyricetin reference standard. These metabolites were presumed to be dominantly generated through hydroxylation, dehydroxylation, methylation, reduction, sulfation, decarbonylation, glucuronidation, glucosylation, and their composite reactions. In a word, our present results not only supplied a solid foundation to better understand the action mechanism of dihydromyricetin, but also provided some models for the metabolism study of the other compounds in traditional Chinese medicines or natural plants.

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