期刊
JOURNAL OF PROTEOME RESEARCH
卷 21, 期 10, 页码 2277-2292出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.2c00133
关键词
proteomics; systems biology; network; heart; pacemaker; cardiomyocytes; epithelial-mesenchymal transition
资金
- AHA [18TPA34170575, 12SDG12060056]
- NIH [R01HL134821]
- NSF [1609831, R01HL111646-01A1]
- Div Of Electrical, Commun & Cyber Sys
- Directorate For Engineering [1609831] Funding Source: National Science Foundation
Research shows that the transcription factor Tbx18 can reprogram ventricular cardiomyocytes into induced pacemaker cells, leading to significant proteome remodeling and electrogenic, metabolic, and cytostructural changes. Additionally, differential expression of proteins associated with the epithelial-to-mesenchymal transition (EMT) was observed, and several transcriptional regulators involved in EMT and heart development were identified.
Previously, we reported that heterologous expression of an embryonic transcription factor, Tbx18, reprograms ventricular cardiomyocytes into induced pacemaker cells (Tbx18-iPMs), though the key pathways are unknown. Here, we have used a tandem mass tag proteomic approach to characterize the impact of Tbx18 on neonatal rat ventricular myocytes. Tbx18 expression triggered vast proteome remodeling. Tbx18-iPMs exhibited increased expression of known pacemaker ion channels, including Hcn4 and Cx45 as well as upregulation of the mechanosensitive ion channels Piezo1, Trpp2 (PKD2), and TrpM7. Metabolic pathways were broadly down-regulated, as were ion channels associated with ventricular excitation-contraction coupling. Tbx18-iPMs also exhibited extensive intracellular cytoskeletal and extracellular matrix remodeling, including 96 differentially expressed proteins associated with the epithelial-to-mesenchymal transition (EMT). RNAseq extended coverage of low abundance transcription factors, revealing upregulation of EMT-inducing Snai1, Snai2, Twist1, Twist2, and Zeb2. Finally, network diffusion mapping of >200 transcriptional regulators indicates EMT and heart development factors occupy adjacent network neighborhoods downstream of Tbx18 but upstream of metabolic control factors. In conclusion, transdifferentiation of cardiac myocytes into pacemaker cells entails massive electrogenic, metabolic, and cytostructural remodeling. Structural changes exhibit hallmarks of the EMT. The results aid ongoing efforts to maximize the yield and phenotypic stability of engineered biological pacemakers.
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